The possible involvement of salicylic acid and hydrogen peroxide in the systemic promotion of phenolic biosynthesis in clover roots colonized by arbuscular mycorrhizal fungus
Copyright © 2015 Elsevier GmbH. All rights reserved.
| Veröffentlicht in: | Journal of plant physiology. - 1979. - 178(2015) vom: 15. Apr., Seite 27-34 |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , , , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2015
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| Zugriff auf das übergeordnete Werk: | Journal of plant physiology |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Arbuscular mycorrhizal fungus Hydrogen peroxide Phenolic accumulation Salicylic acid Systemic induction Phenols Plant Proteins Nitric Oxide mehr... |
| Zusammenfassung: | Copyright © 2015 Elsevier GmbH. All rights reserved. Arbuscular mycorrhizal fungal (AMF) colonization can induce both the local and the systemic increase in phenolic accumulation in hosts. However, the signaling molecules responsible for the systemic induction is still unclear. In this study, a split-root rhizobox system was designed to explore these molecules, with one half of clover (Trifolium repense) roots colonized by AMF, Funneliformis mosseae (formerly known as Glomus mosseae), and the other not (NM/M). Plants with two halves both (M/M) or neither (NM/NM) inoculated were also established for comparison. The contents of phenols and the accumulation of salicylic acid (SA), hydrogen peroxide (H2O2) and nitric oxide (NO) in roots were monitored, the activities of L-phenylalanine ammonia-lyase (PAL) and nitric oxide synthase (NOS) in roots were assayed, and the expressions of pal and chs (gene encoding chalcone synthase) genes in roots were also quantified using qRT-PCR. Results indicated that when phenolic content in NM/NM plants was lower than that in M/M plants, AMF colonization systemically induced the increase in phenolic content in NM/M plants. Similarly, the accumulations of SA and H2O2 were increased by AMF both locally and systemically, while that of NO was only increased locally. Moreover, enzyme assay and qRT-PCR were in accordance with these results. These data suggest that AMF colonization can systemically increase the phenolic biosynthesis, and SA and H2O2 are possibly the signaling molecules involved. The role of MeSA, a signaling molecule capable of long distance transport in this process, is also discussed |
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| Beschreibung: | Date Completed 04.02.2016 Date Revised 30.09.2020 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1618-1328 |
| DOI: | 10.1016/j.jplph.2015.01.016 |