Production of retroviral constructs for effective transfer and expression of T-cell receptor genes using Golden Gate cloning
Here we present an improved strategy for producing T-cell receptor (TCR)-expressing retroviral vectors using a Golden Gate cloning strategy. This method takes advantage of the modular nature of TCR genes by directly amplifying TCR α and β variable regions from RNA or cDNA, then cloning and fusing th...
Ausführliche Beschreibung
Bibliographische Detailangaben
| Veröffentlicht in: | BioTechniques. - 1988. - 58(2015), 3 vom: 15. März, Seite 135-9
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| 1. Verfasser: |
Coren, Lori V
(VerfasserIn) |
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| Weitere Verfasser: |
Jain, Sumiti,
Trivett, Matthew T,
Ohlen, Claes,
Ott, David E |
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| Format: | Online-Aufsatz
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| Sprache: | English |
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| Veröffentlicht: |
2015
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| Zugriff auf das übergeordnete Werk: | BioTechniques
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| Schlagworte: | Journal Article
Research Support, N.I.H., Extramural
Golden Gate cloning
T-cell receptor
gene engineering
retroviral vector
Receptors, Antigen, T-Cell, alpha-beta |
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