Affinity proteomics to study endogenous protein complexes : pointers, pitfalls, preferences and perspectives
Dissecting and studying cellular systems requires the ability to specifically isolate distinct proteins along with the co-assembled constituents of their associated complexes. Affinity capture techniques leverage high affinity, high specificity reagents to target and capture proteins of interest alo...
Ausführliche Beschreibung
Bibliographische Detailangaben
Veröffentlicht in: | BioTechniques. - 1988. - 58(2015), 3 vom: 13. März, Seite 103-19
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1. Verfasser: |
LaCava, John
(VerfasserIn) |
Weitere Verfasser: |
Molloy, Kelly R,
Taylor, Martin S,
Domanski, Michal,
Chait, Brian T,
Rout, Michael P |
Format: | Online-Aufsatz
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Sprache: | English |
Veröffentlicht: |
2015
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Zugriff auf das übergeordnete Werk: | BioTechniques
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Schlagworte: | Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
affinity
interactomics
protein complex
protein purification
proteomics
Multiprotein Complexes
Proteins |