Affinity proteomics to study endogenous protein complexes : pointers, pitfalls, preferences and perspectives

Dissecting and studying cellular systems requires the ability to specifically isolate distinct proteins along with the co-assembled constituents of their associated complexes. Affinity capture techniques leverage high affinity, high specificity reagents to target and capture proteins of interest alo...

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Veröffentlicht in:BioTechniques. - 1988. - 58(2015), 3 vom: 13. März, Seite 103-19
1. Verfasser: LaCava, John (VerfasserIn)
Weitere Verfasser: Molloy, Kelly R, Taylor, Martin S, Domanski, Michal, Chait, Brian T, Rout, Michael P
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2015
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't affinity interactomics protein complex protein purification proteomics Multiprotein Complexes Proteins