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231224s2015 xx |||||o 00| ||eng c |
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|a 10.2144/000114263
|2 doi
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|a pubmed25n0823.xml
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|a DE-627
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|e rakwb
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|a eng
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1 |
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|a Vingataramin, Laurie
|e verfasserin
|4 aut
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| 245 |
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2 |
|a A single protocol for extraction of gDNA from bacteria and yeast
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|c 2015
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 30.11.2015
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|a Date Revised 11.03.2015
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|a published: Electronic-eCollection
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|a Citation Status MEDLINE
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|a Guanidine thiocyanate breakage of microorganisms has been the standard initial step in genomic DNA (gDNA) extraction of microbial DNA for two decades, despite the requirement for pretreatments to extract DNA from microorganisms other than Gram-negative bacteria. We report a quick and low-cost gDNA extraction protocol called EtNa that is efficient for bacteria and yeast over a broad range of concentrations. EtNa is based on a hot alkaline ethanol lysis. The solution can be immediately centrifuged to yield a crude gDNA extract suitable for PCR, or it can be directly applied to a silica column for purification
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4 |
|a Journal Article
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4 |
|a DNA amplification
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|a DNA extraction
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4 |
|a PCR
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| 650 |
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4 |
|a bacteria, yeast
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| 650 |
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7 |
|a DNA, Bacterial
|2 NLM
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| 650 |
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7 |
|a DNA, Fungal
|2 NLM
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| 650 |
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7 |
|a Guanidines
|2 NLM
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| 650 |
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7 |
|a Thiocyanates
|2 NLM
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| 650 |
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7 |
|a guanidine thiocyanate
|2 NLM
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| 650 |
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7 |
|a 593-84-0
|2 NLM
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| 700 |
1 |
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|a Frost, Eric H
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t BioTechniques
|d 1991
|g 58(2015), 3 vom: 25. März, Seite 120-5
|w (DE-627)NLM012627046
|x 1940-9818
|7 nnas
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| 773 |
1 |
8 |
|g volume:58
|g year:2015
|g number:3
|g day:25
|g month:03
|g pages:120-5
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| 856 |
4 |
0 |
|u http://dx.doi.org/10.2144/000114263
|3 Volltext
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|d 58
|j 2015
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|b 25
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|h 120-5
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