Navigating the labyrinth : a guide to sequence-based, community ecology of arbuscular mycorrhizal fungi
© 2015 The Authors. New Phytologist © 2015 New Phytologist Trust.
| Publié dans: | The New phytologist. - 1990. - 207(2015), 1 vom: 20. Juli, Seite 235-247 |
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| Auteur principal: | |
| Autres auteurs: | , , , , , , , , |
| Format: | Article en ligne |
| Langue: | English |
| Publié: |
2015
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| Accès à la collection: | The New phytologist |
| Sujets: | Journal Article Research Support, Non-U.S. Gov't PCR arbuscular mycorrhizal fungi (AMF) bioinformatics environmental sequencing next generation sequencing (NGS) DNA, Fungal |
| Résumé: | © 2015 The Authors. New Phytologist © 2015 New Phytologist Trust. Data generated from next generation sequencing (NGS) will soon comprise the majority of information about arbuscular mycorrhizal fungal (AMF) communities. Although these approaches give deeper insight, analysing NGS data involves decisions that can significantly affect results and conclusions. This is particularly true for AMF community studies, because much remains to be known about their basic biology and genetics. During a workshop in 2013, representatives from seven research groups using NGS for AMF community ecology gathered to discuss common challenges and directions for future research. Our goal was to improve the quality and accessibility of NGS data for the AMF research community. Discussions spanned sampling design, sample preservation, sequencing, bioinformatics and data archiving. With concrete examples we demonstrated how different approaches can significantly alter analysis outcomes. Failure to consider the consequences of these decisions may compound bias introduced at each step along the workflow. The products of these discussions have been summarized in this paper in order to serve as a guide for any researcher undertaking NGS sequencing of AMF communities |
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| Description: | Date Completed 08.04.2016 Date Revised 16.04.2021 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1469-8137 |
| DOI: | 10.1111/nph.13340 |