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231224s2015 xx |||||o 00| ||eng c |
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|a 10.1021/la5044987
|2 doi
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|a DE-627
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|e rakwb
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|a eng
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| 100 |
1 |
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|a Cheng, Fang
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Antibody-ligand interactions for hydrophobic charge-induction chromatography
|b a surface plasmon resonance study
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|c 2015
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|a ƒa Online-Ressource
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|a Date Completed 21.12.2015
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|a Date Revised 24.03.2015
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a This article describes the use of surface plasmon resonance (SPR) spectroscopy to study antibody-ligand interactions for hydrophobic charge-induction chromatography (HCIC) and its versatility in investigating the surface and solution factors affecting the interactions. Two density model surfaces presenting the HCIC ligand (mercapto-ethyl-pyridine, MEP) were prepared on Au using a self-assembly technique. The surface chemistry and structure, ionization, and protein binding of such model surfaces were characterized by X-ray photoelectron spectroscopy (XPS), near-edge X-ray absorption fine structure (NEXAFS), contact-angle titration, and SPR, respectively. The influences of the surface and solution factors, e.g., ligand density, salt concentration, and solution pH, on protein adsorption were determined by SPR. Our results showed that ligand density affects both equilibrium and dynamic aspects of the interactions. Specifically, a dense ligand leads to an increase in binding strength, rapid adsorption, slow desorption, and low specificity. In addition, both hydrophobic interactions and hydrogen bonding contribute significantly to the protein adsorption at neutral pH, while the electrostatic repulsion is overwhelmed under acidic conditions. The hydrophobic interaction at a high concentration of lyotropic salt would cause drastic conformational changes in the adsorbed protein. Combined with the self-assembly technique, SPR proves to be a powerful tool for studying the interactions between an antibody and a chromatographic ligand
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Research Support, U.S. Gov't, Non-P.H.S.
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| 650 |
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|a Ligands
|2 NLM
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| 700 |
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|a Li, Ming-Yang
|e verfasserin
|4 aut
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|a Wang, Han-Qi
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Lin, Dong-Qiang
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Qu, Jing-Ping
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 31(2015), 11 vom: 24. März, Seite 3422-30
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
1 |
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|g volume:31
|g year:2015
|g number:11
|g day:24
|g month:03
|g pages:3422-30
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| 856 |
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|u http://dx.doi.org/10.1021/la5044987
|3 Volltext
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