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231224s2015 xx |||||o 00| ||eng c |
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|a 10.1093/jxb/erv002
|2 doi
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|a pubmed25n0821.xml
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|a (DE-627)NLM246392460
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|a (NLM)25697790
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Koudounas, Konstantinos
|e verfasserin
|4 aut
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|a A defence-related Olea europaea β-glucosidase hydrolyses and activates oleuropein into a potent protein cross-linking agent
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|c 2015
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 27.01.2016
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|a Date Revised 03.12.2021
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|a published: Print-Electronic
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|a GENBANK: AY083162
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|a Citation Status MEDLINE
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|a © The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissionsoup.com.
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|a Oleuropein, the major secoiridoid compound in olive, is involved in a sophisticated two-component defence system comprising a β-glucosidase enzyme that activates oleuropein into a toxic glutaraldehyde-like structure. Although oleuropein deglycosylation studies have been monitored extensively, an oleuropein β-glucosidase gene has not been characterized as yet. Here, we report the isolation of OeGLU cDNA from olive encoding a β-glucosidase belonging to the defence-related group of terpenoid-specific glucosidases. In planta recombinant protein expression assays showed that OeGLU deglycosylated and activated oleuropein into a strong protein cross-linker. Homology and docking modelling predicted that OeGLU has a characteristic (β/α)8 TIM barrel conformation and a typical construction of a pocket-shaped substrate recognition domain composed of conserved amino acids supporting the β-glucosidase activity and non-conserved residues associated with aglycon specificity. Transcriptional analysis in various olive organs revealed that the gene was developmentally regulated, with its transcript levels coinciding well with the spatiotemporal patterns of oleuropein degradation and aglycon accumulation in drupes. OeGLU upregulation in young organs reflects its prominent role in oleuropein-mediated defence system. High gene expression during drupe maturation implies an additional role in olive secondary metabolism, through the degradation of oleuropein and reutilization of hydrolysis products
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a heterologous expression
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|a oleuropein
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|a olive development
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|a plant defence
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|a protein cross-linking.
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|a β-Glucosidase
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|a Iridoid Glucosides
|2 NLM
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|a Iridoids
|2 NLM
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| 650 |
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|a Plant Proteins
|2 NLM
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| 650 |
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|a Terpenes
|2 NLM
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| 650 |
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|a oleuropein
|2 NLM
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| 650 |
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|a 2O4553545L
|2 NLM
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| 650 |
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|a beta-Glucosidase
|2 NLM
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| 650 |
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|a EC 3.2.1.21
|2 NLM
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| 700 |
1 |
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|a Banilas, Georgios
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Michaelidis, Christos
|e verfasserin
|4 aut
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| 700 |
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|a Demoliou, Catherine
|e verfasserin
|4 aut
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| 700 |
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|a Rigas, Stamatis
|e verfasserin
|4 aut
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| 700 |
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|a Hatzopoulos, Polydefkis
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 66(2015), 7 vom: 21. Apr., Seite 2093-106
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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| 773 |
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|g volume:66
|g year:2015
|g number:7
|g day:21
|g month:04
|g pages:2093-106
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| 856 |
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|u http://dx.doi.org/10.1093/jxb/erv002
|3 Volltext
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|a AR
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|d 66
|j 2015
|e 7
|b 21
|c 04
|h 2093-106
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