Comparative interrogation of the developing xylem transcriptomes of two wood-forming species : Populus trichocarpa and Eucalyptus grandis

© 2015 The Authors. New Phytologist © 2015 New Phytologist Trust.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 206(2015), 4 vom: 16. Juni, Seite 1391-405
1. Verfasser: Hefer, Charles A (VerfasserIn)
Weitere Verfasser: Mizrachi, Eshchar, Myburg, Alexander A, Douglas, Carl J, Mansfield, Shawn D
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2015
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Comparative Study Journal Article Research Support, Non-U.S. Gov't Eucalyptus grandis Populus trichocarpa gene expression mRNA-Seq secondary cell wall xylem transcriptome Plant Proteins
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520 |a Wood formation is a complex developmental process governed by genetic and environmental stimuli. Populus and Eucalyptus are fast-growing, high-yielding tree genera that represent ecologically and economically important species suitable for generating significant lignocellulosic biomass. Comparative analysis of the developing xylem and leaf transcriptomes of Populus trichocarpa and Eucalyptus grandis together with phylogenetic analyses identified clusters of homologous genes preferentially expressed during xylem formation in both species. A conserved set of 336 single gene pairs showed highly similar xylem preferential expression patterns, as well as evidence of high functional constraint. Individual members of multi-gene orthologous clusters known to be involved in secondary cell wall biosynthesis also showed conserved xylem expression profiles. However, species-specific expression as well as opposite (xylem versus leaf) expression patterns observed for a subset of genes suggest subtle differences in the transcriptional regulation important for xylem development in each species. Using sequence similarity and gene expression status, we identified functional homologs likely to be involved in xylem developmental and biosynthetic processes in Populus and Eucalyptus. Our study suggests that, while genes involved in secondary cell wall biosynthesis show high levels of gene expression conservation, differential regulation of some xylem development genes may give rise to unique xylem properties 
650 4 |a Comparative Study 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a Eucalyptus grandis 
650 4 |a Populus trichocarpa 
650 4 |a gene expression 
650 4 |a mRNA-Seq 
650 4 |a secondary cell wall 
650 4 |a xylem transcriptome 
650 7 |a Plant Proteins  |2 NLM 
700 1 |a Mizrachi, Eshchar  |e verfasserin  |4 aut 
700 1 |a Myburg, Alexander A  |e verfasserin  |4 aut 
700 1 |a Douglas, Carl J  |e verfasserin  |4 aut 
700 1 |a Mansfield, Shawn D  |e verfasserin  |4 aut 
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