|
|
|
|
| LEADER |
01000naa a22002652 4500 |
| 001 |
NLM246018259 |
| 003 |
DE-627 |
| 005 |
20231224142145.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
231224s2015 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1021/la504771a
|2 doi
|
| 028 |
5 |
2 |
|a pubmed24n0820.xml
|
| 035 |
|
|
|a (DE-627)NLM246018259
|
| 035 |
|
|
|a (NLM)25658965
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Kurniawan, James
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Characterization of solid-supported dipalmitoylphosphatidylcholine membranes containing cholesterol
|
| 264 |
|
1 |
|c 2015
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 24.11.2015
|
| 500 |
|
|
|a Date Revised 03.03.2015
|
| 500 |
|
|
|a published: Print-Electronic
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a The incorporation of cholesterol into dipalmitoylphosphatidylcholine (DPPC) membranes, even in small amounts, has been shown to significantly alter the properties of the membrane. In this work, force-distance interaction profiles of DPPC membranes containing 8 mol % cholesterol obtained using the surface force apparatus are analyzed in the context of high-resolution structural characterization by atomic force microscopy and neutron reflectometry. The adhesion between the mixed membranes was greater than that for pure DPPC and was variable-depending on the number of defects in the outer membrane leaflets. These defects were only detectable by atomic force microscopy and had an average size of 230 ± 30 nm and 1-5% surface density in the outer leaflet. The adhesion between the membranes monotonically increased as the thickness of the membrane decreased-in direct correlation with the number of defects present (exposed hydrophobic groups) in the membrane contact region. Because of the low diffusion rate of gel-phase membranes, the interaction force profiles were stable and no membrane restructuring was observed
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Research Support, U.S. Gov't, Non-P.H.S.
|
| 650 |
|
7 |
|a Lipid Bilayers
|2 NLM
|
| 650 |
|
7 |
|a 1,2-Dipalmitoylphosphatidylcholine
|2 NLM
|
| 650 |
|
7 |
|a 2644-64-6
|2 NLM
|
| 650 |
|
7 |
|a Cholesterol
|2 NLM
|
| 650 |
|
7 |
|a 97C5T2UQ7J
|2 NLM
|
| 700 |
1 |
|
|a Kuhl, Tonya L
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 31(2015), 8 vom: 03. März, Seite 2527-32
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
|
| 773 |
1 |
8 |
|g volume:31
|g year:2015
|g number:8
|g day:03
|g month:03
|g pages:2527-32
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1021/la504771a
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_22
|
| 912 |
|
|
|a GBV_ILN_350
|
| 912 |
|
|
|a GBV_ILN_721
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 31
|j 2015
|e 8
|b 03
|c 03
|h 2527-32
|