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|a 10.5713/ajas.14.0598
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|a eng
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|a Choi, S H
|e verfasserin
|4 aut
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| 245 |
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|a AMPKα, C/EBPβ, CPT1β, GPR43, PPARγ, and SCD Gene Expression in Single- and Co-cultured Bovine Satellite Cells and Intramuscular Preadipocytes Treated with Palmitic, Stearic, Oleic, and Linoleic Acid
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|c 2015
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|a Text
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|a ƒaComputermedien
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|a Date Completed 26.02.2015
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|a Date Revised 30.09.2020
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|a published: Print
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|a Citation Status PubMed-not-MEDLINE
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|a We previously demonstrated that bovine subcutaneous preadipocytes promote adipogenic gene expression in muscle satellite cells in a co-culture system. Herein we hypothesize that saturated fatty acids would promote adipogenic/lipogenic gene expression, whereas mono- and polyunsaturated fatty acids would have the opposite effect. Bovine semimembranosus satellite cells (BSC) and intramuscular preadipocytes (IPA) were isolated from crossbred steers and cultured with 10% fetal bovine serum (FBS)/Dulbecco's Modified Eagle Medium (DMEM) and 1% antibiotics during the 3-d proliferation period. After proliferation, cells were treated for 3 d with 3% horse serum/DMEM (BSC) or 5% FBS/DMEM (IPA) with antibiotics. Media also contained 10 μg/mL insulin and 10 μg/mL pioglitazone. Subsequently, differentiating BSC and IPA were cultured in their respective media with 40 μM palmitic, stearic, oleic, or linoleic acid for 4 d. Finally, BSC and IPA were single- or co-cultured for an additional 2 h. All fatty acid treatments increased (p = 0.001) carnitine palmitoyltransferase-1 beta (CPT1β) gene expression, but the increase in CPT1β gene expression was especially pronounced in IPA incubated with palmitic and stearic acid (6- to 17- fold increases). Oleic and linoleic acid decreased (p = 0.001) stearoyl-CoA desaturase (SCD) gene expression over 80% in both BSC and IPA. Conversely, palmitic and stearic acid increased SCD gene expression three fold in co-cultured in IPA, and stearic acid increased AMPKα gene expression in single- and co-cultured BSC and IPA. Consistent with our hypothesis, saturated fatty acids, especially stearic acid, promoted adipogenic and lipogenic gene expression, whereas unsaturated fatty acids decreased expression of those genes associated with fatty acid metabolism
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|a Journal Article
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|a Bovine
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|a Co-culture
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|a Fatty Acids
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|a Gene Expression
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| 650 |
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|a Preadipocytes
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| 650 |
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|a Satellite Cells
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| 700 |
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|a Park, S K
|e verfasserin
|4 aut
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| 700 |
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|a Johnson, B J
|e verfasserin
|4 aut
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| 700 |
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|a Chung, K Y
|e verfasserin
|4 aut
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| 700 |
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|a Choi, C W
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Kim, K H
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Kim, W Y
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Smith, B
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Asian-Australasian journal of animal sciences
|d 1998
|g 28(2015), 3 vom: 10. März, Seite 411-9
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|g volume:28
|g year:2015
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|g day:10
|g month:03
|g pages:411-9
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|u http://dx.doi.org/10.5713/ajas.14.0598
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