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231224s2015 xx |||||o 00| ||eng c |
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|a 10.5713/ajas.14.0495
|2 doi
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|e rakwb
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|a eng
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|a Yun, Hyeonho
|e verfasserin
|4 aut
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|a Evaluation of Optimum Dietary Threonine Requirement by Plasma Free Threonine and Ammonia Concentrations in Surgically Modified Rainbow Trout, Oncorhynchus mykiss
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|c 2015
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|a Text
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|2 rdacontent
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|a ƒaComputermedien
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|a Date Completed 26.02.2015
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|a Date Revised 30.09.2020
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|a published: Print
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|a Citation Status PubMed-not-MEDLINE
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|a This study was carried out to evaluate the dietary threonine requirement by measuring the plasma free threonine and ammonia concentrations in rainbow trout, Oncorhynchus mykiss after dorsal aorta cannulation. A total of 70 fish (average initial weight 506±8.2 g) were randomly distributed into each of the 14 net cages (5 fish/cage). After 48 hours (h) of feed deprivation, each group was intubated at 1% body weight with one of the seven L-amino acid based diets containing graded levels of threonine (0.42%, 0.72%, 0.92%, 1.12%, 1.32%, 1.52%, or 1.82% of diet, dry matter basis). Blood samples were taken at 0, 5, and 24 h after intubation. Post-prandial plasma free threonine concentrations (PPthr) of fish 5 h after intubation with diets containing 1.32% or more threonine were significantly higher than those of fish intubated with diets containing 1.12% or less threonine (p<0.05). Post-absorptive free threonine concentrations (PAthr) after 24 h of intubation of the fish with diets containing 0.92% or more threonine were significantly higher than those of fish intubated with diets containing 0.72% or less threonine. Post-prandial plasma ammonia concentrations (PPA, 5 h after intubation) were not significantly different among fish intubated with diets containing 1.12% or less threonine, except the PPA of fish intubated with diet containing 0.42% threonine. Broken-line model analyses of PPthr, PAthr, and PPA indicated that the dietary threonine requirement of rainbow trout should be between 0.95% (2.71) and 1.07% (3.06) of diet (% of dietary protein on a dry matter basis)
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|a Journal Article
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|a Dorsal Aorta Cannulation
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|a Plasma
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|a Rainbow Trout
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|a Threonine
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|a Park, Gunjun
|e verfasserin
|4 aut
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|a Ok, Imho
|e verfasserin
|4 aut
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| 700 |
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|a Katya, Kumar
|e verfasserin
|4 aut
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|a Hung, Silas
|e verfasserin
|4 aut
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|a Bai, Sungchul C
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Asian-Australasian journal of animal sciences
|d 1998
|g 28(2015), 4 vom: 10. Apr., Seite 551-8
|w (DE-627)NLM098195883
|x 1011-2367
|7 nnns
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| 773 |
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|g volume:28
|g year:2015
|g number:4
|g day:10
|g month:04
|g pages:551-8
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|u http://dx.doi.org/10.5713/ajas.14.0495
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