Molecular engineering of a PheS counterselection marker for improved operating efficiency in Escherichia coli
Escherichia coli phenylalanyl-tRNA synthetase, a-subunit (ePheS) can be useful as a counterselection marker since its A294G variant misincorporates 4-chloro-phenylalanine (4CP) into cellular proteins during translation, thereby causing cell death. The drawback of this method is that selection must b...
| Veröffentlicht in: | BioTechniques. - 1988. - 58(2015), 2 vom: 21. Feb., Seite 86-8 |
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| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2015
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't 4-chloro-phenylalanine Escherichia coli PheS counterselection phenylalanyl-tRNA synthetase Genetic Markers Mutant Proteins Phenylalanine-tRNA Ligase |
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