Abridged adapter primers increase the target scope of Hi-Plex

Previously, we reported Hi-Plex, an amplicon-based method for targeted massively parallel sequencing capable of generating 60 amplicons simultaneously. In further experiments, however, we found our approach did not scale to higher amplicon numbers. Here, we report a modification to the original Hi-P...

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Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1988. - 58(2015), 1 vom: 16. Jan., Seite 33-6
1. Verfasser: Nguyen-Dumont, Tú (VerfasserIn)
Weitere Verfasser: Hammet, Fleur, Mahmoodi, Maryam, Pope, Bernard J, Giles, Graham G, Hopper, John L, Southey, Melissa C, Park, Daniel J
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2015
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Hi-Plex genetic screening massively parallel sequencing mutation screening next-generation sequencing targeted sequencing DNA Primers mehr... DNA 9007-49-2