|
|
|
|
| LEADER |
01000naa a22002652 4500 |
| 001 |
NLM245481435 |
| 003 |
DE-627 |
| 005 |
20231224140955.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
231224s2015 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1021/la504606x
|2 doi
|
| 028 |
5 |
2 |
|a pubmed24n0818.xml
|
| 035 |
|
|
|a (DE-627)NLM245481435
|
| 035 |
|
|
|a (NLM)25603188
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Hayes, Douglas G
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Effect of protein incorporation on the nanostructure of the bicontinuous microemulsion phase of Winsor-III systems
|b a small-angle neutron scattering study
|
| 264 |
|
1 |
|c 2015
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 26.10.2015
|
| 500 |
|
|
|a Date Revised 17.02.2015
|
| 500 |
|
|
|a published: Print-Electronic
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a Small-angle neutron scattering (SANS) analysis using the Teubner-Strey model has been employed to evaluate the effect of protein incorporation into the middle, bicontinuous microemulsion (BμE) phase of Winsor-III (WIII) systems formed by an aerosol-OT (AOT)/alkyl ethoxylate mixed surfactant system to understand better the extraction of proteins into and out of BμEs and to study the effect of proteins on a system that serves as a biomimetic analog of cell membranes. Under conditions of high salinity, the incorporation of positively charged proteins cytochrome c, lysozyme, and α-chymotrypsin, near their solubilization limit in the BμEs promoted the release of water and oil from the BμEs, a decrease in the quasi-periodic repeat distance (d), an increase in ordering (a decrease in the amphiphilicity factor, fa) for the surfactant monolayers, and a decrease in the surface area per surfactant headgroup, suggesting that the proteins affected the self-assembly of components in the BμE phase and produced Debye shielding of AOT's sulfonate headgroup. For WIII systems possessing lower salinity, cytochrome c reduced the efficiency of surfactant in the BμE phase, noted by increases in d and fa, suggesting that the enzyme and AOT underwent ion pairing. The results of this study demonstrate the importance of ionic strength to modulate protein-surfactant interactions, which in turn will control the release of proteins encapsulated in the BμEs, relevant to WIII-based protein extraction and controlled release from BμE delivery systems, and demonstrate the utility of BμEs as a model system to understand the effect of proteins on biomembranes
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Research Support, U.S. Gov't, Non-P.H.S.
|
| 650 |
|
7 |
|a Aerosols
|2 NLM
|
| 650 |
|
7 |
|a Emulsions
|2 NLM
|
| 650 |
|
7 |
|a Heptanes
|2 NLM
|
| 650 |
|
7 |
|a Oils
|2 NLM
|
| 650 |
|
7 |
|a Proteins
|2 NLM
|
| 650 |
|
7 |
|a Water
|2 NLM
|
| 650 |
|
7 |
|a 059QF0KO0R
|2 NLM
|
| 700 |
1 |
|
|a Gomez del Rio, Javier A
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Ye, Ran
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Urban, Volker S
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Pingali, Sai Venkatesh
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a O'Neill, Hugh M
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 31(2015), 6 vom: 17. Feb., Seite 1901-10
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
|
| 773 |
1 |
8 |
|g volume:31
|g year:2015
|g number:6
|g day:17
|g month:02
|g pages:1901-10
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1021/la504606x
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_22
|
| 912 |
|
|
|a GBV_ILN_350
|
| 912 |
|
|
|a GBV_ILN_721
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 31
|j 2015
|e 6
|b 17
|c 02
|h 1901-10
|