Interaction of a patterned amphiphilic polyphenylene dendrimer with a lipid monolayer : electrostatic interactions dominate
Dendrimeric macromolecules with defined shape and size are promising candidates for delivering drug or DNA molecules into cells. In this work we study the influence of an amphiphilic polyphenylene dendrimer on a model cell membrane consisting of a condensed 1,2-dipalmitoyl-sn-glycero-3-phosphocholin...
| Veröffentlicht in: | Langmuir : the ACS journal of surfaces and colloids. - 1999. - 31(2015), 6 vom: 17. Feb., Seite 1980-7 |
|---|---|
| 1. Verfasser: | |
| Weitere Verfasser: | , , , , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2015
|
| Zugriff auf das übergeordnete Werk: | Langmuir : the ACS journal of surfaces and colloids |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Dendrimers Polymers 1,2-Dipalmitoylphosphatidylcholine 2644-64-6 polyphenylene sulfide 9016-75-5 |
| Zusammenfassung: | Dendrimeric macromolecules with defined shape and size are promising candidates for delivering drug or DNA molecules into cells. In this work we study the influence of an amphiphilic polyphenylene dendrimer on a model cell membrane consisting of a condensed 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) lipid monolayer. A small surface pressure decrease is observed when the dendrimer solution is injected into the aqueous phase below the monolayer. X-ray reflectivity measurements show that the surface monolayer remains intact. The molecular-scale picture is obtained with sum-frequency generation spectroscopy. With this technique, we observe that the tails of the surfactant molecules become less ordered upon interaction with the amphiphilic polyphenylene dendrimer. In contrast, the water molecules below the DPPC layer become more ordered. Our observations suggest that electrostatic interactions between the negative charge of the dendrimer and the positively charged part of the DPPC headgroup keep the dendrimer located below the headgroup. No evidence of dendrimer insertion into the membrane has been observed. Apparently before entering the cell membrane the dendrimer can stick at the hydrophilic part of the lipids |
|---|---|
| Beschreibung: | Date Completed 26.10.2015 Date Revised 17.02.2015 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1520-5827 |
| DOI: | 10.1021/la504252s |