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231224s2017 xx |||||o 00| ||eng c |
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|a 10.1016/j.clim.2015.01.002
|2 doi
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|a pubmed24n1435.xml
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|a DE-627
|b ger
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|e rakwb
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|a eng
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| 100 |
1 |
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|a Ramesh, Manish
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Clonal and constricted T cell repertoire in Common Variable Immune Deficiency
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|c 2017
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 14.08.2017
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|a Date Revised 10.06.2024
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2015 Elsevier Inc. All rights reserved.
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|a We used high throughput sequencing to examine the structure and composition of the T cell receptor β chain in Common Variable Immune Deficiency (CVID). TCRβ CDR3 regions were amplified and sequenced from genomic DNA of 44 adult CVID subjects and 22 healthy adults, using a high-throughput multiplex PCR. CVID TCRs had significantly less junctional diversity, fewer n-nucleotide insertions and deletions, and completely lacked a population of highly modified TCRs, with 13 or more V-gene nucleotide deletions, seen in healthy controls. The CVID CDR3 sequences were significantly more clonal than control DNA, and displayed unique V gene usage. Despite reduced junctional diversity, increased clonality and similar infectious exposures, DNA of CVID subjects shared fewer TCR sequences as compared to controls. These abnormalities are pervasive, found in out-of-frame sequences and thus independent of selection and were not associated with specific clinical complications. These data support an inherent T cell defect in CVID
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|a Journal Article
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|a Research Support, N.I.H., Extramural
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|a Research Support, Non-U.S. Gov't
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| 650 |
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|a Adult
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|a CMV
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| 650 |
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|a Clonality
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| 650 |
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|a Clone sharing
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| 650 |
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|a Common Variable Immune Deficiency
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| 650 |
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|a EBV
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| 650 |
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|a High throughput sequencing
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| 650 |
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|a Junctional diversity
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| 650 |
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4 |
|a T cell receptor
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| 650 |
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|a VDJ recombination
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| 650 |
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|a Complementarity Determining Regions
|2 NLM
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| 650 |
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|a Receptors, Antigen, T-Cell, alpha-beta
|2 NLM
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| 700 |
1 |
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|a Hamm, David
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Simchoni, Noa
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Cunningham-Rundles, Charlotte
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Clinical immunology (Orlando, Fla.)
|d 1999
|g 178(2017) vom: 15. Mai, Seite 1-9
|w (DE-627)NLM098196855
|x 1521-7035
|7 nnns
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| 773 |
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|g volume:178
|g year:2017
|g day:15
|g month:05
|g pages:1-9
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|u http://dx.doi.org/10.1016/j.clim.2015.01.002
|3 Volltext
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