Functional characterization and developmental expression profiling of gibberellin signalling components in Vitis vinifera

Bibliographische Detailangaben
Veröffentlicht in:	Journal of experimental botany. - 1985. - 66(2015), 5 vom: 02. März, Seite 1463-76
1. Verfasser:	Acheampong, Atiako Kwame (VerfasserIn)
Weitere Verfasser:	Hu, Jianhong, Rotman, Ariel, Zheng, Chuanlin, Halaly, Tamar, Takebayashi, Yumiko, Jikumaru, Yusuke, Kamiya, Yuji, Lichter, Amnon, Sun, Tai-Ping, Or, Etti
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2015
Zugriff auf das übergeordnete Werk:	Journal of experimental botany
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. F-box proteins GA receptors VvDELLA proteins. gibberellin signalling gibberellins (GAs) grapevine (Vitis vinifera) Gibberellins mehr... Plant Growth Regulators Plant Proteins

Beschreibung
Zusammenfassung:	© The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology. Gibberellins (GAs) regulate numerous developmental processes in grapevine (Vitis vinifera) such as rachis elongation, fruit set, and fruitlet abscission. The ability of GA to promote berry enlargement has led to its indispensable use in the sternospermocarpic ('seedless') table grape industry worldwide. However, apart from VvGAI1 (VvDELLA1), which regulates internode elongation and fruitfulness, but not berry size of seeded cultivars, little was known about GA signalling in grapevine. We have identified and characterized two additional DELLAs (VvDELLA2 and VvDELLA3), two GA receptors (VvGID1a and VvGID1b), and two GA-specific F-box proteins (VvSLY1a and VvSLY1b), in cv. Thompson seedless. With the exception of VvDELLA3-VvGID1b, all VvDELLAs interacted with the VvGID1s in a GA-dependent manner in yeast two-hybrid assays. Additionally, expression of these grape genes in corresponding Arabidopsis mutants confirmed their functions in planta. Spatiotemporal analysis of VvDELLAs showed that both VvDELLA1 and VvDELLA2 are abundant in most tissues, except in developing fruit where VvDELLA2 is uniquely expressed at high levels, suggesting a key role in fruit development. Our results further suggest that differential organ responses to exogenous GA depend on the levels of VvDELLA proteins and endogenous bioactive GAs. Understanding this interaction will allow better manipulation of GA signalling in grapevine
Beschreibung:	Date Completed 13.01.2016 Date Revised 13.11.2018 published: Print-Electronic Citation Status MEDLINE
ISSN:	1460-2431
DOI:	10.1093/jxb/eru504