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231224s2015 xx |||||o 00| ||eng c |
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7 |
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|a 10.1021/la5040454
|2 doi
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|a pubmed24n0817.xml
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|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Liu, Hu
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Enhanced protein adsorption and facilitated refolding of like-charged protein with highly charged silica nanoparticles fabricated by sequential double modifications
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|c 2015
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 28.09.2015
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|a Date Revised 20.01.2015
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Silica nanoparticles (SNPs) were sequentially modified with poly(ethylenimine) (PEI) and 2-diethylaminoethyl chloride (DEAE) to prepare a series of positively charged SNPs-PEI and SNPs-PEI-DEAE. The sequential double-modification strategy produced a charge density as high as 1740 μmol/g (4524 μmol/mL), which offered a very high adsorption capacity for bovine serum albumin (314 mg/g). Most importantly, the highly charged SNPs-PEI and SNPs-PEI-DEAE could efficiently facilitate the refolding of like-charged protein at extremely low utilization. For instance, in the refolding of 1 mg/mL lysozyme, the refolding yield reached 75% with only 3.3 μL/mL SNPs-PEI-DEAE. The bead consumption was reduced by nearly 96% as compared to that of the charged microspheres used previously to reach a similar yield. The results proved that the polyelectrolyte-modified SNPs were promising for applications in facilitating like-charged protein refolding, and the research opened up a new way for biotechnology applications of highly charged nanoparticles
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Polymers
|2 NLM
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|a Proteins
|2 NLM
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|a Silicon Dioxide
|2 NLM
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| 650 |
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|a 7631-86-9
|2 NLM
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| 650 |
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|a Polyethyleneimine
|2 NLM
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| 650 |
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|a 9002-98-6
|2 NLM
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| 700 |
1 |
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|a Dong, Xiaoyan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Sun, Yan
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 31(2015), 2 vom: 20. Jan., Seite 655-8
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|x 1520-5827
|7 nnns
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| 773 |
1 |
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|g volume:31
|g year:2015
|g number:2
|g day:20
|g month:01
|g pages:655-8
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|u http://dx.doi.org/10.1021/la5040454
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