Functionalized synchrotron in-line phase-contrast computed tomography a novel approach for simultaneous quantification of structural alterations and localization of barium-labelled alveolar macrophages within mouse lung samples

Functionalized synchrotron in-line phase-contrast computed tomography : a novel approach for simultaneous quantification of structural alterations and localization of barium-labelled alveolar macrophages within mouse lung samples

Functionalized computed tomography (CT) in combination with labelled cells is virtually non-existent due to the limited sensitivity of X-ray-absorption-based imaging, but would be highly desirable to realise cell tracking studies in entire organisms. In this study we applied in-line free propagation...

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Veröffentlicht in:Journal of synchrotron radiation. - 1994. - 22(2015), 1 vom: 21. Jan., Seite 143-55
1. Verfasser: Dullin, Christian (VerfasserIn)
Weitere Verfasser: dal Monego, Simeone, Larsson, Emanuel, Mohammadi, Sara, Krenkel, Martin, Garrovo, Chiara, Biffi, Stefania, Lorenzon, Andrea, Markus, Andrea, Napp, Joanna, Salditt, Tim, Accardo, Agostino, Alves, Frauke, Tromba, Giuliana
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2015
Zugriff auf das übergeordnete Werk:Journal of synchrotron radiation
Schlagworte:Journal Article Research Support, Non-U.S. Gov't functional CT imaging phase-contrast CT single-distance phase retrieval Allergens Contrast Media Barium Sulfate 25BB7EKE2E Ovalbumin 9006-59-1
Beschreibung
Zusammenfassung:Functionalized computed tomography (CT) in combination with labelled cells is virtually non-existent due to the limited sensitivity of X-ray-absorption-based imaging, but would be highly desirable to realise cell tracking studies in entire organisms. In this study we applied in-line free propagation X-ray phase-contrast CT (XPCT) in an allergic asthma mouse model to assess structural changes as well as the biodistribution of barium-labelled macrophages in lung tissue. Alveolar macrophages that were barium-sulfate-loaded and fluorescent-labelled were instilled intratracheally into asthmatic and control mice. Mice were sacrificed after 24 h, lungs were kept in situ, inflated with air and scanned utilizing XPCT at the SYRMEP beamline (Elettra Synchrotron Light Source, Italy). Single-distance phase retrieval was used to generate data sets with ten times greater contrast-to-noise ratio than absorption-based CT (in our setup), thus allowing to depict and quantify structural hallmarks of asthmatic lungs such as reduced air volume, obstruction of airways and increased soft-tissue content. Furthermore, we found a higher concentration as well as a specific accumulation of the barium-labelled macrophages in asthmatic lung tissue. It is believe that XPCT will be beneficial in preclinical asthma research for both the assessment of therapeutic response as well as the analysis of the role of the recruitment of macrophages to inflammatory sites
Beschreibung:Date Completed 16.10.2015
Date Revised 10.11.2023
published: Print-Electronic
Citation Status MEDLINE
ISSN:1600-5775
DOI:10.1107/S1600577514021730