|
|
|
|
| LEADER |
01000naa a22002652 4500 |
| 001 |
NLM244794901 |
| 003 |
DE-627 |
| 005 |
20231224135453.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
231224s2015 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1021/la503797w
|2 doi
|
| 028 |
5 |
2 |
|a pubmed24n0816.xml
|
| 035 |
|
|
|a (DE-627)NLM244794901
|
| 035 |
|
|
|a (NLM)25531175
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a DeWitt, Brittany N
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Interaction of cholesterol in ternary lipid mixtures investigated using single-molecule fluorescence
|
| 264 |
|
1 |
|c 2015
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 30.09.2015
|
| 500 |
|
|
|a Date Revised 16.11.2017
|
| 500 |
|
|
|a published: Print-Electronic
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a Fluorescence measurements of the sterol analog 23-(dipyrrometheneboron difluoride)-24-norcholesterol (BODIPY-cholesterol) are used to compare the effects of cholesterol (Chol) in monolayers of 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC)/1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC)/Chol and chicken egg sphingomyelin (SM)/DOPC/Chol. Monolayers are formed using the Langmuir-Blodgett technique and compared at surface pressures of 8 and 30 mN/m. In particular, these ternary lipid mixtures are compared using both ensemble and single-molecule fluorescence measurements of BODIPY-cholesterol. In mixed monolayers incorporating 0.10 mol % BODIPY-cholesterol, fluorescence microscopy measurements as a function of cholesterol added reveal similar trends in monolayer phase structure for both DPPC/DOPC/Chol and SM/DOPC/Chol films. With a probe concentration reduced to ∼10(-8) mol % BODIPY-cholesterol, single-molecule fluorescence measurements using defocused polarized total internal reflection microscopy are used to characterize the orientations of BODIPY-cholesterol in the monolayers. Population histograms of the BODIPY emission dipole tilt angle away from the membrane normal reveal distinct insertion geometries with a preferred angle observed near 78°. The measured angles and populations are relatively insensitive to added cholesterol and changes in surface pressure for monolayers of SM/DOPC/Chol. For monolayers of DPPC/DOPC/Chol, however, the single-molecule measurements reveal significant changes in the BODIPY-cholesterol insertion geometry when the surface pressure is increased to 30 mN/m. These changes are discussed in terms of a squeeze-out mechanism for BODIPY-cholesterol in these monolayers and provide insight into the partitioning and arrangement of BODIPY-cholesterol in ternary lipid mixtures
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Research Support, Non-U.S. Gov't
|
| 650 |
|
7 |
|a 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene
|2 NLM
|
| 650 |
|
7 |
|a Boron Compounds
|2 NLM
|
| 650 |
|
7 |
|a Fluorescent Dyes
|2 NLM
|
| 650 |
|
7 |
|a Phosphatidylcholines
|2 NLM
|
| 650 |
|
7 |
|a Phosphatidylethanolamines
|2 NLM
|
| 650 |
|
7 |
|a Sphingomyelins
|2 NLM
|
| 650 |
|
7 |
|a Xanthenes
|2 NLM
|
| 650 |
|
7 |
|a 1,2-Dipalmitoylphosphatidylcholine
|2 NLM
|
| 650 |
|
7 |
|a 2644-64-6
|2 NLM
|
| 650 |
|
7 |
|a 1,2-dipalmitoyl-3-phosphatidylethanolamine
|2 NLM
|
| 650 |
|
7 |
|a 3026-45-7
|2 NLM
|
| 650 |
|
7 |
|a Texas red
|2 NLM
|
| 650 |
|
7 |
|a 82354-19-6
|2 NLM
|
| 650 |
|
7 |
|a Cholesterol
|2 NLM
|
| 650 |
|
7 |
|a 97C5T2UQ7J
|2 NLM
|
| 650 |
|
7 |
|a 1,2-oleoylphosphatidylcholine
|2 NLM
|
| 650 |
|
7 |
|a EDS2L3ODLV
|2 NLM
|
| 700 |
1 |
|
|a Dunn, Robert C
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 31(2015), 3 vom: 27. Jan., Seite 995-1004
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
|
| 773 |
1 |
8 |
|g volume:31
|g year:2015
|g number:3
|g day:27
|g month:01
|g pages:995-1004
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1021/la503797w
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_22
|
| 912 |
|
|
|a GBV_ILN_350
|
| 912 |
|
|
|a GBV_ILN_721
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 31
|j 2015
|e 3
|b 27
|c 01
|h 995-1004
|