Differential gene expression and alternative splicing between diploid and tetraploid watermelon

Bibliographische Detailangaben
Veröffentlicht in:	Journal of experimental botany. - 1985. - 66(2015), 5 vom: 15. März, Seite 1369-85
1. Verfasser:	Saminathan, Thangasamy (VerfasserIn)
Weitere Verfasser:	Nimmakayala, Padma, Manohar, Sumanth, Malkaram, Sridhar, Almeida, Aldo, Cantrell, Robert, Tomason, Yan, Abburi, Lavanya, Rahman, Mohammad A, Vajja, Venkata G, Khachane, Amit, Kumar, Brajendra, Rajasimha, Harsha K, Levi, Amnon, Wehner, Todd, Reddy, Umesh K
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2015
Zugriff auf das übergeordnete Werk:	Journal of experimental botany
Schlagworte:	Comparative Study Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. RACE-PCR RNA-seq alternative splicing gene expression genome duplication mehr... tetraploid watermelon (Citrullus lanatus). Plant Proteins

Beschreibung
Zusammenfassung:	© The Author 2014. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissionsoup.com. The exploitation of synthetic polyploids for producing seedless fruits is well known in watermelon. Tetraploid progenitors of triploid watermelon plants, compared with their diploid counterparts, exhibit wide phenotypic differences. Although many factors modulate alternative splicing (AS) in plants, the effects of autopolyploidization on AS are still unknown. In this study, we used tissues of leaf, stem, and fruit of diploid and tetraploid sweet watermelon to understand changes in gene expression and the occurrence of AS. RNA-sequencing analysis was performed along with reverse transcription quantitative PCR and rapid amplification of cDNA ends (RACE)-PCR to demonstrate changes in expression and splicing. All vegetative tissues except fruit showed an increased level of AS in the tetraploid watermelon throughout the growth period. The ploidy levels of diploids and the tetraploid were confirmed using a ploidy analyser. We identified 5362 and 1288 genes that were up- and downregulated, respectively, in tetraploid as compared with diploid plants. We further confirmed that 22 genes underwent AS events across tissues, indicating possibilities of generating different protein isoforms with altered functions of important transcription factors and transporters. Arginine biosynthesis, chlorophyllide synthesis, GDP mannose biosynthesis, trehalose biosynthesis, and starch and sucrose degradation pathways were upregulated in autotetraploids. Phloem protein 2, chloroplastic PGR5-like protein, zinc-finger protein, fructokinase-like 2, MYB transcription factor, and nodulin MtN21 showed AS in fruit tissues. These results should help in developing high-quality seedless watermelon and provide additional transcriptomic information related to other cucurbits
Beschreibung:	Date Completed 13.01.2016 Date Revised 09.04.2022 published: Print-Electronic Citation Status MEDLINE
ISSN:	1460-2431
DOI:	10.1093/jxb/eru486