Automation of the cytokinesis-block micronucleus cytome assay by laser scanning cytometry and its potential application in radiation biodosimetry

Here we describe the adaptation of laser scanning cytometry (LSC) to measure micronuclei (MN) automatically in lymphocytes. MN frequencies were determined in irradiated human lymphocytes using the cytokinesis-block technique, and the results from LSC were compared with visual scoring results obtaine...

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Veröffentlicht in:BioTechniques. - 1993. - 57(2014), 6 vom: 11. Dez., Seite 309-12
1. Verfasser: François, Maxime (VerfasserIn)
Weitere Verfasser: Hochstenbach, Kevin, Leifert, Wayne, Fenech, Michael Felix
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2014
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Micronucleus assay automation laser scanning cytometry Indoles DAPI 47165-04-8