Assessing and reducing sources of gene expression variability in Staphylococcus epidermidis biofilms

Gene expression quantification can be a useful tool in studying the properties of bacterial biofilms. Unfortunately, techniques such as RNA extraction, cDNA synthesis, and quantitative PCR (qPCR) can introduce variability into mRNA transcript measurements, obscuring biologically relevant results. He...

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Publié dans:	BioTechniques. - 1991. - 57(2014), 6 vom: 11. Dez., Seite 295-301
Auteur principal:	Sousa, Cármen (Auteur)
Autres auteurs:	França, Angela, Cerca, Nuno
Format:	Article en ligne
Langue:	English
Publié:	2014
Accès à la collection:	BioTechniques
Sujets:	Journal Article Research Support, Non-U.S. Gov't RNA extraction biofilm pool biofilms gene expression variability qPCR reverse transcriptase DNA, Bacterial RNA, Bacterial RNA, Messenger