Assessing and reducing sources of gene expression variability in Staphylococcus epidermidis biofilms
Gene expression quantification can be a useful tool in studying the properties of bacterial biofilms. Unfortunately, techniques such as RNA extraction, cDNA synthesis, and quantitative PCR (qPCR) can introduce variability into mRNA transcript measurements, obscuring biologically relevant results. He...
Ausführliche Beschreibung
Bibliographische Detailangaben
| Veröffentlicht in: | BioTechniques. - 1991. - 57(2014), 6 vom: 11. Dez., Seite 295-301
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| 1. Verfasser: |
Sousa, Cármen
(VerfasserIn) |
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| Weitere Verfasser: |
França, Angela,
Cerca, Nuno |
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| Format: | Online-Aufsatz
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| Sprache: | English |
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| Veröffentlicht: |
2014
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| Zugriff auf das übergeordnete Werk: | BioTechniques
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| Schlagworte: | Journal Article
Research Support, Non-U.S. Gov't
RNA extraction
biofilm pool
biofilms
gene expression variability
qPCR
reverse transcriptase
DNA, Bacterial
RNA, Bacterial
RNA, Messenger |
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