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231224s2015 xx |||||o 00| ||eng c |
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|a 10.1016/j.plaphy.2014.12.001
|2 doi
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|a pubmed24n0814.xml
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|a (DE-627)NLM244389012
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|a (NLM)25486023
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|a (PII)S0981-9428(14)00364-7
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Fernández, María Belén
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Isolation and characterization of a Solanum tuberosum subtilisin-like protein with caspase-3 activity (StSBTc-3)
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|c 2015
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 17.09.2015
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|a Date Revised 07.12.2022
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2014 Elsevier Masson SAS. All rights reserved.
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|a Plant proteases with caspase-like enzymatic activity have been widely studied during the last decade. Previously, we have reported the presence and induction of caspase-3 like activity in the apoplast of potato leaves during Solanum tuberosum- Phytophthora infestans interaction. In this work we have purified and identified a potato extracellular protease with caspase-3 like enzymatic activity from potato leaves infected with P. infestans. Results obtained from the size exclusion chromatography show that the isolated protease is a monomeric enzyme with an estimated molecular weight of 70 kDa approximately. Purified protease was analyzed by MALDI-TOF MS, showing a 100% of sequence identity with the deduced amino acid sequence of a putative subtilisin-like protease from S. tuberosum (Solgenomics protein ID: PGSC0003DMP400018521). For this reason the isolated protease was named as StSBTc-3. This report constitutes the first evidence of isolation and identification of a plant subtilisin-like protease with caspase-3 like enzymatic activity. In order to elucidate the possible function of StSBTc-3 during plant pathogen interaction, we demonstrate that like animal caspase-3, StSBTc-3 is able to produce in vitro cytoplasm shrinkage in plant cells and to induce plant cell death. This result suggest that, StSBTc-3 could exert a caspase executer function during potato- P. infestans interaction, resulting in the restriction of the pathogen spread during plant-pathogen interaction
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Apoplast
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|a DEVDase
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| 650 |
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|a Plant–pathogen interaction
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| 650 |
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|a Solanum tuberosum
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|a Subtilisin like protein
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| 650 |
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|a Plant Proteins
|2 NLM
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| 650 |
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|a Subtilisin
|2 NLM
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| 650 |
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|a EC 3.4.21.62
|2 NLM
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| 650 |
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|a Caspase 3
|2 NLM
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| 650 |
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7 |
|a EC 3.4.22.-
|2 NLM
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| 700 |
1 |
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|a Daleo, Gustavo Raúl
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Guevara, María Gabriela
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 86(2015) vom: 08. Jan., Seite 137-146
|w (DE-627)NLM098178261
|x 1873-2690
|7 nnns
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| 773 |
1 |
8 |
|g volume:86
|g year:2015
|g day:08
|g month:01
|g pages:137-146
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| 856 |
4 |
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|u http://dx.doi.org/10.1016/j.plaphy.2014.12.001
|3 Volltext
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