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231224s2015 xx |||||o 00| ||eng c |
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|a 10.1016/j.jplph.2014.09.014
|2 doi
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|a pubmed24n0813.xml
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|a (DE-627)NLM244170223
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|a (NLM)25462979
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|a (PII)S0176-1617(14)00279-X
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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1 |
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|a Chojnacka, Magdalena
|e verfasserin
|4 aut
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|a A triticale water-deficit-inducible phytocystatin inhibits endogenous cysteine proteinases in vitro
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|c 2015
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 03.08.2015
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2014 Elsevier GmbH. All rights reserved.
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|a Water-deficit is accompanied by an increase in proteolysis. Phytocystatins are plant inhibitors of cysteine proteinases that belong to the papain and legumain family. A cDNA encoding the protein inhibitor TrcC-8 was identified in the vegetative organs of triticale. In response to water-deficit, increases in the mRNA levels of TrcC-8 were observed in leaf and root tissues. Immunoblot analysis indicated that accumulation of the TrcC-8 protein occurred after 72h of water-deficit in the seedlings. Using recombinant protein, inhibitory activity of TrcC-8 against cysteine proteases from triticale and wheat tissues was analyzed. Under water-deficit conditions, there are increases in cysteine proteinase activities in both plant tissues. The cysteine proteinase activities were inhibited by addition of the recombinant TrcC-8 protein. These results suggest a potential role for the triticale phytocystatin in modulating cysteine proteinase activities during water-deficit conditions
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Cysteine proteinase
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|a Drought stress
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|a Phytocystatin
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|a Water deficit
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|a Cystatins
|2 NLM
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|a Plant Proteins
|2 NLM
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| 650 |
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|a Water
|2 NLM
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|a 059QF0KO0R
|2 NLM
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|a Cysteine Proteases
|2 NLM
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| 650 |
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|a EC 3.4.-
|2 NLM
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| 700 |
1 |
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|a Szewińska, Joanna
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Mielecki, Marcin
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Nykiel, Małgorzata
|e verfasserin
|4 aut
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| 700 |
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|a Imai, Ryozo
|e verfasserin
|4 aut
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| 700 |
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|a Bielawski, Wiesław
|e verfasserin
|4 aut
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| 700 |
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|a Orzechowski, Sławomir
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of plant physiology
|d 1979
|g 174(2015) vom: 01. Feb., Seite 161-5
|w (DE-627)NLM098174622
|x 1618-1328
|7 nnns
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| 773 |
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|g volume:174
|g year:2015
|g day:01
|g month:02
|g pages:161-5
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|u http://dx.doi.org/10.1016/j.jplph.2014.09.014
|3 Volltext
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|d 174
|j 2015
|b 01
|c 02
|h 161-5
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