Overexpression of wheat NF-YA10 gene regulates the salinity stress response in Arabidopsis thaliana
Copyright © 2014 Elsevier Masson SAS. All rights reserved.
| Veröffentlicht in: | Plant physiology and biochemistry : PPB. - 1991. - 86(2015) vom: 03. Jan., Seite 34-43 |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , , , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2015
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| Zugriff auf das übergeordnete Werk: | Plant physiology and biochemistry : PPB |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Abscisic acid CCAAT-box NF-Y transcription factor SR3 Salinity stress Wheat Arabidopsis Proteins CCAAT-Binding Factor mehr... |
| Zusammenfassung: | Copyright © 2014 Elsevier Masson SAS. All rights reserved. The nuclear factor Y (NF-Y) transcription factor is formed by the interaction of three distinct subunits (NF-YA, -YB and -YC). It targets the CCAAT box, a common cis-element in eukaryotic promoters. Here, the bread wheat gene TaNF-YA10-1 has been isolated from the salinity tolerant cultivar SR3. Recombinant TaNF-YA10-1 was heterologously produced in Escherichia coli, and the purified protein successfully bound to the CCAAT motif in vitro. TaNF-YA10-1 was down-regulated by the imposition of salinity and abscisic acid (ABA). The constitutive expression of TaNF-YA10-1 in Arabidopsis thaliana significantly increased the plant's sensitivity to salinity and repressed its sensitivity to ABA as judged from the seed germination, cotyledon greening and the relative root growth. The transcription of stress-related genes AtRAB18, AtRD29B, AtABI5, AtCBF1 and AtCBF3 was downregulated in TaNF-YA10-1 overexpression transgenic plants. The data provide supportive evidence that TaNFYA10-1 is involved in the regulation of growth under salinity stress conditions |
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| Beschreibung: | Date Completed 17.09.2015 Date Revised 09.04.2022 published: Print-Electronic GENBANK: KM983028 Citation Status MEDLINE |
| ISSN: | 1873-2690 |
| DOI: | 10.1016/j.plaphy.2014.11.011 |