Effects of UV-B radiation on anatomical characteristics, phenolic compounds and gene expression of the phenylpropanoid pathway in highbush blueberry leaves

The effects of increased doses of UV-B radiation on anatomical, biochemical and molecular features of leaves of two highbush blueberry (Vaccinium corymbosum L. cv. Brigitta and Bluegold) genotypes were investigated. Plants were grown in a solid substrate and exposed to 0, 0.07, 0.12 and 0.19 Wm(-2)...

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Veröffentlicht in:Plant physiology and biochemistry : PPB. - 1991. - 85(2014) vom: 28. Dez., Seite 85-95
1. Verfasser: Inostroza-Blancheteau, Claudio (VerfasserIn)
Weitere Verfasser: Reyes-Díaz, Marjorie, Arellano, Alejandro, Latsague, Mirtha, Acevedo, Patricio, Loyola, Rodrigo, Arce-Johnson, Patricio, Alberdi, Miren
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2014
Zugriff auf das übergeordnete Werk:Plant physiology and biochemistry : PPB
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Phenols Propionates
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520 |a The effects of increased doses of UV-B radiation on anatomical, biochemical and molecular features of leaves of two highbush blueberry (Vaccinium corymbosum L. cv. Brigitta and Bluegold) genotypes were investigated. Plants were grown in a solid substrate and exposed to 0, 0.07, 0.12 and 0.19 Wm(-2) of biologically effective UV-B radiation for up to 72 h. Leaf thickness and the adaxial epidermis thickness fell more than 3-fold in both genotypes at the highest UV-B dose. Moreover, in Bluegold an evident disorganization in the different cell layers was observed at the highest UV-B radiation. A significant decrease in chlorophyll a/b after 6 h in Brigitta under the greater UV-B doses was observed. Anthocyanin and total phenolics were increased, especially at 0.19 Wm(-2), when compared to the control in both genotypes.Chlorogenic acid was the most abundant hydroxycinnamic acid in Brigitta, and was significantly higher (P ≤ 0.05) than in Bluegold leaves. Regarding the expression of phenylpropanoid genes, only the transcription factor VcMYBPA1 showed a significant and sustained induction at higher doses of UV-B radiation in both genotypes compared to the controls. Thus, the reduction of leaf thickness concomitant with a lower lipid peroxidation and rapid enhancement of secondary metabolites, accompanied by a stable induction of the VcMYBPA1 transcription factor suggest a better performance against UV-B radiation of the Brigitta genotype 
650 4 |a Journal Article 
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700 1 |a Reyes-Díaz, Marjorie  |e verfasserin  |4 aut 
700 1 |a Arellano, Alejandro  |e verfasserin  |4 aut 
700 1 |a Latsague, Mirtha  |e verfasserin  |4 aut 
700 1 |a Acevedo, Patricio  |e verfasserin  |4 aut 
700 1 |a Loyola, Rodrigo  |e verfasserin  |4 aut 
700 1 |a Arce-Johnson, Patricio  |e verfasserin  |4 aut 
700 1 |a Alberdi, Miren  |e verfasserin  |4 aut 
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