Overexpression of codA gene confers enhanced tolerance to abiotic stresses in alfalfa

We generated transgenic alfalfa plants (Medicago sativa L. cv. Xinjiang Daye) expressing a bacterial codA gene in chloroplasts under the control of the SWPA2 promoter (referred to as SC plants) and evaluated the plants under various abiotic stress conditions. Three transgenic plants (SC7, SC8, and S...

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Veröffentlicht in:Plant physiology and biochemistry : PPB. - 1991. - 85(2014) vom: 19. Dez., Seite 31-40
1. Verfasser: Li, Hongbing (VerfasserIn)
Weitere Verfasser: Wang, Zhi, Ke, Qingbo, Ji, Chang Yoon, Jeong, Jae Cheol, Lee, Haeng-Soon, Lim, Yong Pyo, Xu, Bingcheng, Deng, Xi-Ping, Kwak, Sang-Soo
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2014
Zugriff auf das übergeordnete Werk:Plant physiology and biochemistry : PPB
Schlagworte:Journal Article Research Support, Non-U.S. Gov't
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520 |a We generated transgenic alfalfa plants (Medicago sativa L. cv. Xinjiang Daye) expressing a bacterial codA gene in chloroplasts under the control of the SWPA2 promoter (referred to as SC plants) and evaluated the plants under various abiotic stress conditions. Three transgenic plants (SC7, SC8, and SC9) were selected for further characterization based on the strong expression levels of codA in response to methylviologen (MV)-mediated oxidative stress. SC plants showed enhanced tolerance to NaCl and drought stress on the whole plant level due to induced expression of codA. When plants were subjected to 250 mM NaCl treatment for 2 weeks, SC7 and SC8 plants maintained higher chlorophyll contents and lower malondialdehyde levels than non-transgenic (NT) plants. Under drought stress conditions, all SC plants showed enhanced tolerance to drought stress through maintaining high relative water contents and increased levels of glycinebetaine and proline compared to NT plants. Under normal conditions, SC plants exhibited increased growth due to increased expression of auxin-related IAA genes compared to NT plants. These results suggest that the SC plants generated in this study will be useful for enhanced biomass production on global marginal lands, such as high salinity and arid lands, yielding a sustainable agricultural product 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
700 1 |a Wang, Zhi  |e verfasserin  |4 aut 
700 1 |a Ke, Qingbo  |e verfasserin  |4 aut 
700 1 |a Ji, Chang Yoon  |e verfasserin  |4 aut 
700 1 |a Jeong, Jae Cheol  |e verfasserin  |4 aut 
700 1 |a Lee, Haeng-Soon  |e verfasserin  |4 aut 
700 1 |a Lim, Yong Pyo  |e verfasserin  |4 aut 
700 1 |a Xu, Bingcheng  |e verfasserin  |4 aut 
700 1 |a Deng, Xi-Ping  |e verfasserin  |4 aut 
700 1 |a Kwak, Sang-Soo  |e verfasserin  |4 aut 
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