DNA profiles from fingermarks

Criminal investigations would be considerably improved if DNA profiles could be routinely generated from single fingermarks. Here we report a direct DNA profiling method that was able to generate interpretable profiles from 71% of 170 fingermarks. The data are based on fingermarks from all 5 digits...

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Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1993. - 57(2014), 5 vom: 01. Nov., Seite 259-66
1. Verfasser: Templeton, Jennifer E L (VerfasserIn)
Weitere Verfasser: Linacre, Adrian
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2014
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article DNA profiling NGM SElect Profiler Plus direct PCR fingermarks short tandem repeats
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520 |a Criminal investigations would be considerably improved if DNA profiles could be routinely generated from single fingermarks. Here we report a direct DNA profiling method that was able to generate interpretable profiles from 71% of 170 fingermarks. The data are based on fingermarks from all 5 digits of 34 individuals. DNA was obtained from the fingermarks using a swab moistened with Triton-X, and the fibers were added directly to one of two commercial DNA profiling kits. All profiles were obtained without increasing the number of amplification cycles; therefore, our method is ideally suited for adoption by the forensic science community. We indicate the use of the technique in a criminal case in which a DNA profile was generated from a fingermark on tape that was wrapped around a drug seizure. Our direct DNA profiling approach is rapid and able to generate profiles from touched items when current forensic practices have little chance of success 
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