High-resolution genetic maps of Eucalyptus improve Eucalyptus grandis genome assembly
© 2014 CIRAD. New Phytologist © 2014 New Phytologist Trust.
Veröffentlicht in: | The New phytologist. - 1979. - 206(2015), 4 vom: 11. Juni, Seite 1283-96 |
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1. Verfasser: | |
Weitere Verfasser: | , , , , , , , |
Format: | Online-Aufsatz |
Sprache: | English |
Veröffentlicht: |
2015
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Zugriff auf das übergeordnete Werk: | The New phytologist |
Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Eucalyptus genetic mapping genome assembly segregation distortion single nucleotide polymorphism (SNP) array Genetic Markers |
Zusammenfassung: | © 2014 CIRAD. New Phytologist © 2014 New Phytologist Trust. Genetic maps are key tools in genetic research as they constitute the framework for many applications, such as quantitative trait locus analysis, and support the assembly of genome sequences. The resequencing of the two parents of a cross between Eucalyptus urophylla and Eucalyptus grandis was used to design a single nucleotide polymorphism (SNP) array of 6000 markers evenly distributed along the E. grandis genome. The genotyping of 1025 offspring enabled the construction of two high-resolution genetic maps containing 1832 and 1773 markers with an average marker interval of 0.45 and 0.5 cM for E. grandis and E. urophylla, respectively. The comparison between genetic maps and the reference genome highlighted 85% of collinear regions. A total of 43 noncollinear regions and 13 nonsynthetic regions were detected and corrected in the new genome assembly. This improved version contains 4943 scaffolds totalling 691.3 Mb of which 88.6% were captured by the 11 chromosomes. The mapping data were also used to investigate the effect of population size and number of markers on linkage mapping accuracy. This study provides the most reliable linkage maps for Eucalyptus and version 2.0 of the E. grandis genome |
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Beschreibung: | Date Completed 01.02.2016 Date Revised 16.03.2022 published: Print-Electronic Citation Status MEDLINE |
ISSN: | 1469-8137 |
DOI: | 10.1111/nph.13150 |