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231224s2014 xx |||||o 00| ||eng c |
| 024 |
7 |
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|a 10.1021/la503858t
|2 doi
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|a pubmed24n0810.xml
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|a DE-627
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|e rakwb
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|a eng
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| 100 |
1 |
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|a Roeder, Sebastian
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Silica entrapment for significantly stabilized, energy-conducting light-harvesting complex (LHCII)
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|c 2014
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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| 500 |
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|a Date Completed 15.07.2015
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|a Date Revised 02.12.2014
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a The major light-harvesting chlorophyll a/b complex (LHCII) of the photosynthetic apparatus in green plants consists of a membrane protein and numerous noncovalently bound pigments that make up about one-third of the molecular mass of the pigment-protein complex. Due to this high pigment density, LHCII is potentially interesting as a light-harvesting component in synthetic constructs. However, for such applications its stability needs to be significantly improved. In this work, LHCII was dramatically stabilized by enclosing it within polymerizing colloidal silica. The entrapped LHCII stayed functional at 50 °C for up to 24 h instead of a few minutes in detergent solution and clearly showed energy transfer between complexes. Entrapment yield was enhanced by a polycationic peptide attached to the N terminus. Both the extent of stabilization and the yield of entrapment strongly increased with decreasing diameters of the silica particles
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|a Journal Article
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|a Light-Harvesting Protein Complexes
|2 NLM
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| 650 |
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|a Photosystem II Protein Complex
|2 NLM
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| 650 |
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|a Silicon Dioxide
|2 NLM
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| 650 |
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|a 7631-86-9
|2 NLM
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| 700 |
1 |
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|a Hobe, Stephan
|e verfasserin
|4 aut
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|a Paulsen, Harald
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 30(2014), 47 vom: 02. Dez., Seite 14234-40
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
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|g volume:30
|g year:2014
|g number:47
|g day:02
|g month:12
|g pages:14234-40
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|u http://dx.doi.org/10.1021/la503858t
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