Quantitative and dynamic measurements of biological fresh samples with X-ray phase contrast tomography
X-ray phase contrast tomography using a Talbot grating interferometer was applied to biological fresh samples which were not fixed by any fixatives. To achieve a high-throughput measurement for the fresh samples the X-ray phase contrast tomography measurement procedure was improved. The three-dimens...
Veröffentlicht in: | Journal of synchrotron radiation. - 1994. - 21(2014), Pt 6 vom: 01. Nov., Seite 1347-57 |
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Weitere Verfasser: | , , |
Format: | Online-Aufsatz |
Sprache: | English |
Veröffentlicht: |
2014
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Zugriff auf das übergeordnete Werk: | Journal of synchrotron radiation |
Schlagworte: | Comparative Study Journal Article X-ray phase contrast tomography dynamic imaging quantitative imaging three-dimensional densitometry Contrast Media |
Zusammenfassung: | X-ray phase contrast tomography using a Talbot grating interferometer was applied to biological fresh samples which were not fixed by any fixatives. To achieve a high-throughput measurement for the fresh samples the X-ray phase contrast tomography measurement procedure was improved. The three-dimensional structure of a fresh mouse fetus was clearly depicted as a mass density map using X-ray phase contrast tomography. The mouse fetus measured in the fresh state was then fixed by formalin and measured in the fixed state. The influence of the formalin fixation on soft tissue was quantitatively evaluated by comparing the fresh and fixed samples. X-ray phase contrast tomography was also applied to the dynamic measurement of a biological fresh sample. Morphological changes of a ring-shaped fresh pig aorta were measured tomographically under different degrees of stretching |
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Beschreibung: | Date Completed 20.10.2015 Date Revised 21.10.2021 published: Print-Electronic Citation Status MEDLINE |
ISSN: | 1600-5775 |
DOI: | 10.1107/S1600577514018128 |