Rice nitrate transporter OsNPF2.4 functions in low-affinity acquisition and long-distance transport

© The Author 2014. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissionsoup.com.

Bibliographische Detailangaben
Veröffentlicht in:Journal of experimental botany. - 1985. - 66(2015), 1 vom: 20. Jan., Seite 317-31
1. Verfasser: Xia, Xiudong (VerfasserIn)
Weitere Verfasser: Fan, Xiaorong, Wei, Jia, Feng, Huimin, Qu, Hongye, Xie, Dan, Miller, Anthony J, Xu, Guohua
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2015
Zugriff auf das übergeordnete Werk:Journal of experimental botany
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Growth reduction low-affinity nitrate transporter mutant overexpression potassium recycling rice. Anion Transport Proteins Nitrate Transporters mehr... Nitrates Potassium RWP5GA015D
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520 |a Plant proteins belonging to the NPF (formerly NRT1/PTR) family are well represented in every genome and function in transporting a wide variety of substrates. In this study, we showed that rice OsNPF2.4 is located in the plasma membrane and is expressed mainly in the epidermis, xylem parenchyma, and phloem companion cells. Functional analysis in oocytes showed that OsNPF2.4 is a pH-dependent, low-affinity NO₃⁻ transporter. Short-term (¹⁵NO₃⁻) influx rate, long-term NO₃⁻ acquisition by root, and upward transfer from root to shoot were decreased by disruption of OsNPF2.4 and increased by OsNPF2.4 overexpression under high NO₃⁻ supply. Moreover, the redistribution of NO₃⁻ in the mutants in comparison with the wild type from the oldest leaf to other organs, particularly to N-starved roots, was dramatically changed. Knockout of OsNPF2.4 decreased rice growth and potassium (K) concentration in xylem sap, root, culm, and sheath, but increased the shoot:root ratio of tissue K under higher NO₃⁻. We conclude that OsNPF2.4 functions in acquisition and long-distance transport of NO₃⁻ , and that altering its expression has an indirect effect on K recycling between the root and shoot 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a Growth reduction 
650 4 |a low-affinity nitrate transporter 
650 4 |a mutant 
650 4 |a overexpression 
650 4 |a potassium recycling 
650 4 |a rice. 
650 7 |a Anion Transport Proteins  |2 NLM 
650 7 |a Nitrate Transporters  |2 NLM 
650 7 |a Nitrates  |2 NLM 
650 7 |a Potassium  |2 NLM 
650 7 |a RWP5GA015D  |2 NLM 
700 1 |a Fan, Xiaorong  |e verfasserin  |4 aut 
700 1 |a Wei, Jia  |e verfasserin  |4 aut 
700 1 |a Feng, Huimin  |e verfasserin  |4 aut 
700 1 |a Qu, Hongye  |e verfasserin  |4 aut 
700 1 |a Xie, Dan  |e verfasserin  |4 aut 
700 1 |a Miller, Anthony J  |e verfasserin  |4 aut 
700 1 |a Xu, Guohua  |e verfasserin  |4 aut 
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