Succinimidyl ester surface chemistry : implications of the competition between aminolysis and hydrolysis on covalent protein immobilization

N-Hydroxysuccinimide (NHS) ester terminal groups are commonly used to covalently couple amine-containing biomolecules (e.g., proteins and peptides) to surfaces via amide linkages. This one-step aminolysis is often performed in buffered aqueous solutions near physiological pH (pH 6 to pH 9). Under th...

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Veröffentlicht in:	Langmuir : the ACS journal of surfaces and colloids. - 1992. - 30(2014), 43 vom: 04. Nov., Seite 12868-78
1. Verfasser:	Lim, China Y (VerfasserIn)
Weitere Verfasser:	Owens, Nicholas A, Wampler, Ronald D, Ying, Yixin, Granger, Jennifer H, Porter, Marc D, Takahashi, Makoto, Shimazu, Katsuaki
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2014
Zugriff auf das übergeordnete Werk:	Langmuir : the ACS journal of surfaces and colloids
Schlagworte:	Journal Article Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S. Amines Borates Buffers Esters Immobilized Proteins Succinimides N-hydroxysuccinimide MJE3791M4T