Suppression of xylan endotransglycosylase PtxtXyn10A affects cellulose microfibril angle in secondary wall in aspen wood

Suppression of xylan endotransglycosylase PtxtXyn10A affects cellulose microfibril angle in secondary wall in aspen wood

© 2014 The Authors. New Phytologist © 2014 New Phytologist Trust.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 205(2015), 2 vom: 12. Jan., Seite 666-81
1. Verfasser: Derba-Maceluch, Marta (VerfasserIn)
Weitere Verfasser: Awano, Tatsuya, Takahashi, Junko, Lucenius, Jessica, Ratke, Christine, Kontro, Inkeri, Busse-Wicher, Marta, Kosik, Ondrej, Tanaka, Ryo, Winzéll, Anders, Kallas, Åsa, Leśniewska, Joanna, Berthold, Fredrik, Immerzeel, Peter, Teeri, Tuula T, Ezcurra, Ines, Dupree, Paul, Serimaa, Ritva, Mellerowicz, Ewa J
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2015
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Populus endotransglycosylase growth stresses hybrid aspen secondary cell wall wood formation xylan xylanase mehr... Plant Proteins Xylans Cellulose 9004-34-6 Xylosidases EC 3.2.1.-
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520 |a Certain xylanases from family GH10 are highly expressed during secondary wall deposition, but their function is unknown. We carried out functional analyses of the secondary-wall specific PtxtXyn10A in hybrid aspen (Populus tremula × tremuloides). PtxtXyn10A function was analysed by expression studies, overexpression in Arabidopsis protoplasts and by downregulation in aspen. PtxtXyn10A overexpression in Arabidopsis protoplasts resulted in increased xylan endotransglycosylation rather than hydrolysis. In aspen, the enzyme was found to be proteolytically processed to a 68 kDa peptide and residing in cell walls. Its downregulation resulted in a corresponding decrease in xylan endotransglycosylase activity and no change in xylanase activity. This did not alter xylan molecular weight or its branching pattern but affected the cellulose-microfibril angle in wood fibres, increased primary growth (stem elongation, leaf formation and enlargement) and reduced the tendency to form tension wood. Transcriptomes of transgenic plants showed downregulation of tension wood related genes and changes in stress-responsive genes. The data indicate that PtxtXyn10A acts as a xylan endotransglycosylase and its main function is to release tensional stresses arising during secondary wall deposition. Furthermore, they suggest that regulation of stresses in secondary walls plays a vital role in plant development 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a Populus 
650 4 |a endotransglycosylase 
650 4 |a growth stresses 
650 4 |a hybrid aspen 
650 4 |a secondary cell wall 
650 4 |a wood formation 
650 4 |a xylan 
650 4 |a xylanase 
650 7 |a Plant Proteins  |2 NLM 
650 7 |a Xylans  |2 NLM 
650 7 |a Cellulose  |2 NLM 
650 7 |a 9004-34-6  |2 NLM 
650 7 |a Xylosidases  |2 NLM 
650 7 |a EC 3.2.1.-  |2 NLM 
700 1 |a Awano, Tatsuya  |e verfasserin  |4 aut 
700 1 |a Takahashi, Junko  |e verfasserin  |4 aut 
700 1 |a Lucenius, Jessica  |e verfasserin  |4 aut 
700 1 |a Ratke, Christine  |e verfasserin  |4 aut 
700 1 |a Kontro, Inkeri  |e verfasserin  |4 aut 
700 1 |a Busse-Wicher, Marta  |e verfasserin  |4 aut 
700 1 |a Kosik, Ondrej  |e verfasserin  |4 aut 
700 1 |a Tanaka, Ryo  |e verfasserin  |4 aut 
700 1 |a Winzéll, Anders  |e verfasserin  |4 aut 
700 1 |a Kallas, Åsa  |e verfasserin  |4 aut 
700 1 |a Leśniewska, Joanna  |e verfasserin  |4 aut 
700 1 |a Berthold, Fredrik  |e verfasserin  |4 aut 
700 1 |a Immerzeel, Peter  |e verfasserin  |4 aut 
700 1 |a Teeri, Tuula T  |e verfasserin  |4 aut 
700 1 |a Ezcurra, Ines  |e verfasserin  |4 aut 
700 1 |a Dupree, Paul  |e verfasserin  |4 aut 
700 1 |a Serimaa, Ritva  |e verfasserin  |4 aut 
700 1 |a Mellerowicz, Ewa J  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t The New phytologist  |d 1979  |g 205(2015), 2 vom: 12. Jan., Seite 666-81  |w (DE-627)NLM09818248X  |x 1469-8137  |7 nnns 
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