An experimental means of transmitting pancreas disease in Atlantic salmon Salmo salar L. fry in freshwater

Bibliographische Detailangaben
Veröffentlicht in:	Journal of fish diseases. - 1998. - 38(2015), 3 vom: 08. März, Seite 271-81
1. Verfasser:	Cano, I (VerfasserIn)
Weitere Verfasser:	Joiner, C, Bayley, A, Rimmer, G, Bateman, K, Feist, S W, Stone, D, Paley, R
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2015
Zugriff auf das übergeordnete Werk:	Journal of fish diseases
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't RT-PCR bath infection histology in situ hybridisation salmon pancreas disease salmonid alphavirus

Beschreibung
Zusammenfassung:	© 2014 Crown Copyright. Journal of Fish Diseases © 2014 John Wiley & Sons Ltd. A challenge model for pancreas disease in Atlantic salmon, Salmo salar L. fry, was developed comparing two salmonid alphavirus (SAV) subtypes: SAV1 and SAV5. Viral doses of 3 × 10(5) TCID50 mL(-1) for SAV1 and 3 × 10(4) for SAV5 were tested in triplicate tanks, each containing 450 salmon fry. Cumulative mortalities of 1.2% were recorded. Titres of virus recovered from the mortalities ranged from 10(2) to 10(7) TCID50 mL(-1) . Fry were sampled at 3, 5 and 7.5 weeks post-challenge. Sampling after 3 weeks revealed a high prevalence of infection in the absence of clinical signs, and infectious virus was recovered from 80% and 43% of sampled fry infected with SAV1 and SAV5, respectively. After 5 weeks pancreas, heart and red skeletal muscle lesions were generally observed, whilst degeneration in white skeletal muscle was observed only in fish infected with SAV1. In situ hybridisation confirmed the presence of viral genome in infected pancreas, heart and muscle. After 7.5 weeks, infectious virus (both isolates) was recovered from 13.3% of the fish sampled, with a viral titre of 10(2) TCID50 mL(-1) . Clearly, salmon fry are susceptible to SAV infection and pancreas disease
Beschreibung:	Date Completed 11.05.2015 Date Revised 30.09.2020 published: Print-Electronic Citation Status MEDLINE
ISSN:	1365-2761
DOI:	10.1111/jfd.12310