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231224s2014 xx |||||o 00| ||eng c |
| 024 |
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|a 10.1021/la503444g
|2 doi
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|a pubmed24n0808.xml
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|a (NLM)25291048
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Wang, Lin
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Efficient gene delivery and multimodal imaging by lanthanide-based upconversion nanoparticles
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|c 2014
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 29.06.2015
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|a Date Revised 04.11.2014
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Nanoparticles have been explored as nonviral gene carriers for years because of the simplicity of surface modification and lack of immune response. Lanthanide-based upconversion nanoparticles (UCNPs) are becoming attractive candidates for biomedical applications in virtue of their unique optical properties and multimodality imaging ability. Here, we report a UCNPs-based structure with polyethylenimine coating for both efficient gene transfection and trimodality imaging. Cytotoxicity tests demonstrated that the nanoparticles exhibited significantly decreased cytotoxicity compared to polyethylenimine polymer. Further, in vitro studies revealed that the gene carriers are able to transfer the enhanced green fluorescence protein (EGFP) plasmid DNA into Hela cells in higher transfection efficiency than PEI. Gene silencing was also examined by delivering bcl-2 siRNA into Hela cells, resulting in significant downregulation of target bcl-2 mRNA. More importantly, we demonstrated the feasibility of upconversion gene carriers to serve as effective contrast agents for MRI/CT/UCL trimodality imaging both in vitro and in vivo. The facile fabrication process, great biocompatibility, enhanced gene transfection efficiency, and great bioimaging ability can make it promising for application in gene therapy
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4 |
|a Journal Article
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|a Research Support, Non-U.S. Gov't
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| 650 |
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7 |
|a Drug Carriers
|2 NLM
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| 650 |
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|a Lanthanoid Series Elements
|2 NLM
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| 650 |
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|a Proto-Oncogene Proteins c-bcl-2
|2 NLM
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| 650 |
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|a RNA, Small Interfering
|2 NLM
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| 650 |
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7 |
|a enhanced green fluorescent protein
|2 NLM
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| 650 |
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|a Green Fluorescent Proteins
|2 NLM
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| 650 |
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|a 147336-22-9
|2 NLM
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| 650 |
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|a Polyethyleneimine
|2 NLM
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| 650 |
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|a 9002-98-6
|2 NLM
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| 700 |
1 |
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|a Liu, Jianhua
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Dai, Yunlu
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Yang, Qiang
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhang, Yuanxin
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Yang, Piaoping
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Cheng, Ziyong
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Lian, Hongzhou
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Li, Chunxia
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Hou, Zhiyao
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Ma, Ping'an
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Lin, Jun
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 30(2014), 43 vom: 04. Nov., Seite 13042-51
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
1 |
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|g volume:30
|g year:2014
|g number:43
|g day:04
|g month:11
|g pages:13042-51
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| 856 |
4 |
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|u http://dx.doi.org/10.1021/la503444g
|3 Volltext
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