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231224s2014 xx |||||o 00| ||eng c |
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|a 10.1021/la501453h
|2 doi
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|a eng
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|a Chae, Su-Kyoung
|e verfasserin
|4 aut
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|a Simple fabrication method for a porous poly(vinyl alcohol) matrix by multisolvent mixtures for an air-exposed model of the lung epithelial system
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|c 2014
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|a Text
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|a ƒaComputermedien
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|a ƒa Online-Ressource
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|a Date Completed 15.06.2015
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|a Date Revised 31.03.2022
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a We introduce a simple and easy method for fabricating a thin and porous matrix that can be used as an extracellular matrix (ECM). A porous poly(vinyl alcohol) (PVA) matrix was created through recrystallization by multiple solvents under distilled water (DW), isopropyl alcohol (IPA), and a combination of DW and IPA. The crysatllization was driven by precipitating and dissolving a solute in a solution of a solvent and a nonsolvent, which induced the formation of microspheres in the IPA. The crystal structure depended on the ratio of the solvent/nonsolvent and the concentration of the PVA aqueous solution; these properties were used to tune the thickness, size, and porosity of the matrices. The resulting PVA matrix was chemically stabilized through a reaction with glutaraldehyde in the IPA solution. We demonstrated that a very thin and porous PVA matrix provided an effective functional model of the lung epithelial system. Lung epithelial cells (A549) displayed a high affinity for this matrix, which was permeable to the culture medium. These properties facilitated culturing under the air environment
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Solvents
|2 NLM
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|a Polyvinyl Alcohol
|2 NLM
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|a 9002-89-5
|2 NLM
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|a Mun, Cho Hay
|e verfasserin
|4 aut
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|a Noh, Da-Yoon
|e verfasserin
|4 aut
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|a Kang, Edward
|e verfasserin
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|a Lee, Sang-Hoon
|e verfasserin
|4 aut
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 30(2014), 41 vom: 21. Okt., Seite 12107-13
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|x 1520-5827
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|g volume:30
|g year:2014
|g number:41
|g day:21
|g month:10
|g pages:12107-13
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|u http://dx.doi.org/10.1021/la501453h
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