A wheat 1-FEH w3 variant underlies enzyme activity for stem WSC remobilization to grain under drought

© 2014 The Authors. New Phytologist © 2014 New Phytologist Trust.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 205(2015), 1 vom: 21. Jan., Seite 293-305
1. Verfasser: Zhang, Jingjuan (VerfasserIn)
Weitere Verfasser: Xu, Yunji, Chen, Wei, Dell, Bernard, Vergauwen, Rudy, Biddulph, Ben, Khan, Nusrat, Luo, Hao, Appels, Rudi, Van den Ende, Wim
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2015
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Research Support, Non-U.S. Gov't fructan degradation gene expression grain filling single nucleotide polymorphism (SNP) stem water-soluble carbohydrate (WSC) remobilization terminal drought Carbohydrates Fructans mehr... Genetic Markers Plant Proteins Water 059QF0KO0R Fructose 30237-26-4
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520 |a In wheat stems, the levels of fructan-dominated water-soluble carbohydrates (WSC) do not always correlate well with grain yield. Field drought experiments were carried out to further explain this lack of correlation. Wheat (Triticum aestivum) varieties, Westonia, Kauz and c. 20 genetically diverse double haploid (DH) lines derived from them were investigated. Substantial genotypic differences in fructan remobilization were found and the 1-FEH w3 gene was shown to be the major contributor in the stem fructan remobilization process based on enzyme activity and gene expression results. A single nucleotide polymorphism (SNP) was detected in an auxin response element in the 1-FEH w3 promoter region, therefore we speculated that the mutated Westonia allele might affect gene expression and enzyme activity levels. A cleaved amplified polymorphic (CAP) marker was generated from the SNP. The harvested results showed that the mutated Westonia 1-FEH w3 allele was associated with a higher thousand grain weight (TGW) under drought conditions in 2011 and 2012. These results indicated that higher gene expression of 1-FEH w3 and 1-FEH w3 mediated enzyme activities that favoured stem WSC remobilization to the grains. The CAP marker residing in the 1-FEH w3 promoter region may facilitate wheat breeding by selecting lines with high stem fructan remobilization capacity under terminal drought 
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650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a fructan degradation 
650 4 |a gene expression 
650 4 |a grain filling 
650 4 |a single nucleotide polymorphism (SNP) 
650 4 |a stem water-soluble carbohydrate (WSC) remobilization 
650 4 |a terminal drought 
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700 1 |a Xu, Yunji  |e verfasserin  |4 aut 
700 1 |a Chen, Wei  |e verfasserin  |4 aut 
700 1 |a Dell, Bernard  |e verfasserin  |4 aut 
700 1 |a Vergauwen, Rudy  |e verfasserin  |4 aut 
700 1 |a Biddulph, Ben  |e verfasserin  |4 aut 
700 1 |a Khan, Nusrat  |e verfasserin  |4 aut 
700 1 |a Luo, Hao  |e verfasserin  |4 aut 
700 1 |a Appels, Rudi  |e verfasserin  |4 aut 
700 1 |a Van den Ende, Wim  |e verfasserin  |4 aut 
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773 1 8 |g volume:205  |g year:2015  |g number:1  |g day:21  |g month:01  |g pages:293-305 
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