Plasma membrane and actin cytoskeleton as synergistic barriers to nanowire cell penetration

Nanowires are a rapidly emerging platform for manipulation of and material delivery directly into the cell cytosol. These high aspect ratio structures can breach the lipid membrane; however, the yield of penetrant structures is low, and the mechanism is largely unknown. In particular, some nanostruc...

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Publié dans:Langmuir : the ACS journal of surfaces and colloids. - 1985. - 30(2014), 41 vom: 21. Okt., Seite 12362-7
Auteur principal: Aalipour, Amin (Auteur)
Autres auteurs: Xu, Alexander M, Leal-Ortiz, Sergio, Garner, Craig C, Melosh, Nicholas A
Format: Article en ligne
Langue:English
Publié: 2014
Accès à la collection:Langmuir : the ACS journal of surfaces and colloids
Sujets:Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Cobalt 3G0H8C9362
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520 |a Nanowires are a rapidly emerging platform for manipulation of and material delivery directly into the cell cytosol. These high aspect ratio structures can breach the lipid membrane; however, the yield of penetrant structures is low, and the mechanism is largely unknown. In particular, some nanostructures appear to defeat the membrane transiently, while others can retain long-term access. Here, we examine if local dissolution of the lipid membrane, actin cytoskeleton, or both can enhance nanowire penetration. It is possible that, during cell contact, membrane rupture occurs; however, if the nanostructures do not penetrate the cytoskeleton, the membrane may reclose over a relatively short time frame. We show with quantitative analysis of the number of penetrating nanowires that the lipid bilayer and actin cytoskeleton are synergistic barriers to nanowire cell access, yet chemical poration through both is still insufficient to increase long-term access for adhered cells 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a Research Support, U.S. Gov't, Non-P.H.S. 
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700 1 |a Xu, Alexander M  |e verfasserin  |4 aut 
700 1 |a Leal-Ortiz, Sergio  |e verfasserin  |4 aut 
700 1 |a Garner, Craig C  |e verfasserin  |4 aut 
700 1 |a Melosh, Nicholas A  |e verfasserin  |4 aut 
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