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231224s2014 xx |||||o 00| ||eng c |
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|a 10.1016/j.plaphy.2014.08.021
|2 doi
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|a pubmed25n0806.xml
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|a (DE-627)NLM242011128
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|a (NLM)25232679
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|a (PII)S0981-9428(14)00268-X
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
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|a Wang, Qiu Jun
|e verfasserin
|4 aut
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| 245 |
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|a Cloning and characterization of an elicitor-responsive gene encoding 3-hydroxy-3-methylglutaryl coenzyme A reductase involved in 20-hydroxyecdysone production in cell cultures of Cyanotis arachnoidea
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|c 2014
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 20.08.2015
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|a Date Revised 18.03.2022
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2014 Elsevier Masson SAS. All rights reserved.
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|a Cyanotis arachnoidea contains a rich source of bioactive phytoecdysteroids (i.e. analogues of insect steroid hormones). 3-Hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) supplies mevalonate for the synthesis of many secondary metabolites including 20-hydroxyecdysone (20E), one of metabolism-enhancing phytoecdysteroids. In this study, in order to develop a sustainable source of 20E, cell suspension cultures were established from shoot cultures of C. arachnoidea, and a full length cDNA encoding HMGR (designated as CaHMGR) was cloned and characterized. The cDNA contained 2037 nucleotides with a complete open reading frame (ORF) of 1800 nucleotides, which was predicted to encode a peptide of 599 amino acids. Expression analysis by real-time PCR revealed that CaHMGR mRNA was abundant in C. arachnoidea stems, roots and leaves. When cultivated in Murashige & Skoog medium supplemented with 0.2 mg L(-1) 1-naphthlcetic acid (NAA) and 3.0 mg L(-1) 6-benzyladenine (6-BA), C. arachnoidea cells in suspension culture grew rapidly, yielding 20E (124.14 μg L(-1)) after 12 days. The content of 20E in cell cultures elicited by 0.2 mM methyl jasmonate (MeJA), 100 mg L(-1) yeast elicitor (YE) or 25 μM AgNO3 was increased 8-, 2-, and 6-fold over the control, respectively. Quantitative real-time PCR analysis showed that CaHMGR was expressed at a higher level under the treatment of MeJA or Ag(+) elicitor. Our results suggested that 20E accumulation may be the result of the expression up-regulation of CaHMGR involved in the biosynthesis under the treatment of various elicitors
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a 20-Hydroxyecdysterone
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|a 3-Hydroxy-3-methylglutaryl coenzyme A reductase
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|a Cyanotis arachnoidea
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|a Elicitation
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|a Gene expression
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|a Acetates
|2 NLM
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|a Acyl Coenzyme A
|2 NLM
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|a Carboxylic Acids
|2 NLM
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|a Cyclopentanes
|2 NLM
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|a Naphthalenes
|2 NLM
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|a Oxylipins
|2 NLM
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|a 3-hydroxy-3-methylglutaryl-coenzyme A
|2 NLM
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|a 1553-55-5
|2 NLM
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|a 1-naphthoic acid
|2 NLM
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|a 2NIV4O66BH
|2 NLM
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|a Ecdysterone
|2 NLM
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|a 5289-74-7
|2 NLM
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|a methyl jasmonate
|2 NLM
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| 650 |
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|a 900N171A0F
|2 NLM
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| 700 |
1 |
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|a Zheng, Li Ping
|e verfasserin
|4 aut
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1 |
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|a Zhao, Pei Fei
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhao, Yi Lu
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wang, Jian Wen
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 84(2014) vom: 18. Nov., Seite 1-9
|w (DE-627)NLM098178261
|x 1873-2690
|7 nnns
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| 773 |
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|g volume:84
|g year:2014
|g day:18
|g month:11
|g pages:1-9
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|u http://dx.doi.org/10.1016/j.plaphy.2014.08.021
|3 Volltext
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|d 84
|j 2014
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|h 1-9
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