Isolation of urinary extracellular vesicles from Tamm- Horsfall protein-depleted urine and their application in the development of a lectin-exosome-binding assay

Urine is a readily available source of relatively large quantities of extracellular vesicles (EVs). However, the isolation of urinary EVs (uEVs) is complicated by the presence of Tamm-Horsfall protein (THP), which polymerizes and co-precipitates as a contaminant. This may make glycan analysis of uEV...

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Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1991. - 57(2014), 3 vom: 11., Seite 143-9
1. Verfasser: Kosanović, Maja (VerfasserIn)
Weitere Verfasser: Janković, Miroslava
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2014
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article Research Support, Non-U.S. Gov't THP exosomes extracellular vesicles isolation lectin-exosome-binding assay urine Lectins Uromodulin