Precise gene deletion and replacement using the CRISPR/Cas9 system in human cells

The prokaryotic type II CRISPR/Cas9 system has been adapted to perform targeted genome editing in cells and model organisms. Here, we describe targeted gene deletion and replacement in human cells via the CRISPR/Cas9 system using two guide RNAs. The system effectively generated targeted deletions of...

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Bibliographische Detailangaben

Veröffentlicht in:	BioTechniques. - 1988. - 57(2014), 3 vom: 11., Seite 115-24
1. Verfasser:	Zheng, Qiupeng (VerfasserIn)
Weitere Verfasser:	Cai, Xiaohong, Tan, Meng How, Schaffert, Steven, Arnold, Christopher P, Gong, Xue, Chen, Chang-Zheng, Huang, Shenglin
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2014
Zugriff auf das übergeordnete Werk:	BioTechniques
Schlagworte:	Journal Article Research Support, N.I.H., Extramural CRISPR/Cas9 Genome editing deletion replacement