MPK6 controls H2 O2-induced root elongation by mediating Ca2+ influx across the plasma membrane of root cells in Arabidopsis seedlings

© 2014 The Authors. New Phytologist © 2014 New Phytologist Trust.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 205(2015), 2 vom: 22. Jan., Seite 695-706
1. Verfasser: Han, Shuan (VerfasserIn)
Weitere Verfasser: Fang, Lin, Ren, Xuejian, Wang, Wenle, Jiang, Jing
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2015
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Research Support, Non-U.S. Gov't AtMPK6 abscisic acid (ABA) apoplastic H2O2 increase cell wall peroxidase34 cytosolic Ca2+ rise plasma membrane Ca2+ channels root elongation Arabidopsis Proteins mehr... Abscisic Acid 72S9A8J5GW Hydrogen Peroxide BBX060AN9V Peroxidases EC 1.11.1.- PRX34 protein, Arabidopsis EC 1.11.1.7 MPK6 protein, Arabidopsis EC 2.7.11.24 Mitogen-Activated Protein Kinases Calcium SY7Q814VUP
Beschreibung
Zusammenfassung:© 2014 The Authors. New Phytologist © 2014 New Phytologist Trust.
Mitogen-activated protein kinases (MPKs) play critical roles in signalling and growth, and Ca(2+) and H2 O2 control plant growth processes associated with abscisic acid (ABA). However, it remains unclear how MPKs are involved in H2 O2 - and Ca(2+) -mediated root elongation. Root elongation in seedlings of the loss-of-function mutant Atmpk6 (Arabidopsis thaliana MPK6) was less sensitive to moderate H2 O2 or ABA than that in wild-type (WT) plants. The enhanced elongation was a result of root cell expansion. This effect disappeared when ABA-induced H2 O2 accumulation or the cytosolic Ca(2+) increase were defective. Molecular and biochemical evidence showed that increased expression of the cell wall peroxidase PRX34 in Atmpk6 root cells enhanced apoplastic H2 O2 generation; this promoted a cytosolic Ca(2+) increase and Ca(2+) influx across the plasma membrane. The plasma membrane damage caused by high levels of H2 O2 was ameliorated in a Ca(2+) -dependent manner. These results suggested that there was intensified PRX34-mediated H2 O2 generation in the apoplast and increased Ca(2+) flux into the cytosol of Atmpk6 root cells; that is, the spatial separation of apoplastic H2 O2 from cytosolic Ca(2+) in root cells prevented H2 O2 -induced inhibition of root elongation in Atmpk6 seedlings
Beschreibung:Date Completed 24.08.2015
Date Revised 30.09.2020
published: Print-Electronic
Citation Status MEDLINE
ISSN:1469-8137
DOI:10.1111/nph.12990