Automated hematologic analysis of bone marrow aspirate samples from healthy Beagle dogs

Bibliographische Detailangaben
Veröffentlicht in:	Veterinary clinical pathology. - 1975. - 43(2014), 3 vom: 07. Sept., Seite 342-51
1. Verfasser:	Tan, E (VerfasserIn)
Weitere Verfasser:	Abrams-Ogg, A C G, Defarges, A, Bienzle, D
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2014
Zugriff auf das übergeordnete Werk:	Veterinary clinical pathology
Schlagworte:	Evaluation Study Journal Article Advia dog erythroid hematopoiesis myeloid

Beschreibung
Zusammenfassung:	© 2014 American Society for Veterinary Clinical Pathology and European Society for Veterinary Clinical Pathology. BACKGROUND: Interpretation of bone marrow (BM) smears typically is comprised of qualitative assessment and differential counting of cells. Analysis of BM fluid with automated hematology analyzers may provide rapid characterization of cells to supplement microscopic interpretation OBJECTIVES: The purpose of the study was to examine the practicality and utility of analyzing BM samples in the Advia 2120 hematology analyzer; to determine if results correlate with smear assessment; and to establish descriptive statistics from hematologically normal and clinically healthy Beagle dogs METHODS: Anticoagulated BM aspirates from 3 different sites of 26 adult Beagle dogs were collected. BM samples were analyzed in the Advia 2120, and numerical results were correlated with microscopic assessment of corresponding BM smears. Results from automated analyses and manual 500-cell differential counts were statistically analyzed RESULTS: Forty-six samples were suitable for complete analysis. Results were available in approximately 2 (Advia) and 30 (stained and cover-slipped smear) minutes. Advia nucleated cell concentration was significantly correlated with microscopic assessment of smear particle number and smear cellularity. Significant correlations were also identified for Advia percent neutrophils with segmented, band and metamylocyte neutrophils, Advia percent lymphocytes with rubricytes, and Advia percent large unstained cells (LUC) with myeloblasts and promyelocytes CONCLUSIONS: Automated analysis of BM aspirates was practicable, although techniques to obtain cellular samples and avoid clot formation could be improved. Automated analysis may provide rapid and useful preliminary information regarding sample cellularity, and granulocytic and erythrocytic components. Automated analysis should not supplant microscopic assessment, but may be a useful adjunct
Beschreibung:	Date Completed 12.06.2015 Date Revised 10.12.2019 published: Print-Electronic Citation Status MEDLINE
ISSN:	1939-165X
DOI:	10.1111/vcp.12175