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231224s2014 xx |||||o 00| ||eng c |
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|a 10.1016/j.plaphy.2014.07.013
|2 doi
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|a eng
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|a Christeller, John T
|e verfasserin
|4 aut
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|a α-linolenic acid concentration and not wounding per se is the key regulator of octadecanoid (oxylipin) pathway activity in rice (Oryza sativa L.) leaves
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|c 2014
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|a Text
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|2 rdacontent
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|a ƒaComputermedien
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|a ƒa Online-Ressource
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|a Date Completed 03.12.2015
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2014 Elsevier Masson SAS. All rights reserved.
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|a Using an in vitro system composed of crushed leaf tissues to simulate the wounding response in rice leaves, we established that synthesis of jasmonic acid (JA) and jasmonic acid-isoleucine (JA-Ile) can only occur in unwounded tissue and, in wounded tissue, that only the chloroplast-located section of the octadecanoid pathway is active, resulting in the accumulation of 12-oxo-phytodienoic acid (OPDA). We further showed that OPDA accumulation in vitro was inhibited by 90% using the general lipase inhibitor, tetrahydrolipstatin, indicating that production of α-linolenic acid was the rate-limiting step in octadecanoid pathway activity in rice following wounding and the enzyme capacity for an active pathway was already present. We confirmed this result by showing that added α-linolenic acid stimulated OPDA synthesis in vitro and stimulated OPDA, JA and JA-Ile synthesis in vivo in unwounded tissue. Thus, the response to wounding can be mimicked by the provision of free α-linolenic acid. Our results draw attention to the key importance of lipase activity in initiation of JA and JA-Ile biosynthesis and our lack of knowledge of the cognate lipase(s), lipase substrate identity and mechanism(s) of activation in wounded and unwounded tissue
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a 12-oxo-phytodienoic acid
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|a Jasmonic acid
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|a Octadecanoid pathway
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|a Oryza sativa
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|a α-Linolenic acid
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|a Oxylipins
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|a alpha-Linolenic Acid
|2 NLM
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|a Galis, Ivan
|e verfasserin
|4 aut
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|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 83(2014) vom: 16. Okt., Seite 117-25
|w (DE-627)NLM098178261
|x 1873-2690
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|g volume:83
|g year:2014
|g day:16
|g month:10
|g pages:117-25
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|u http://dx.doi.org/10.1016/j.plaphy.2014.07.013
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