Carbon monoxide regulates the expression of the wound-inducible gene ipomoelin through antioxidation and MAPK phosphorylation in sweet potato

© The Author 2014. Published by Oxford University Press on behalf of the Society for Experimental Biology.

Bibliographische Detailangaben
Veröffentlicht in:Journal of experimental botany. - 1985. - 65(2014), 18 vom: 01. Okt., Seite 5279-90
1. Verfasser: Lin, Jeng-Shane (VerfasserIn)
Weitere Verfasser: Lin, Hsin-Hung, Li, Yu-Chi, King, Yu-Chi, Sung, Ruei-Jin, Kuo, Yun-Wei, Lin, Chih-Ching, Shen, Yu-Hsing, Jeng, Shih-Tong
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2014
Zugriff auf das übergeordnete Werk:Journal of experimental botany
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Carbon monoxide ERK phosphorylation H2O2 ipomoelin sweet potato wounding. Plant Proteins ipomoelin protein, Ipomoea batatas mehr... Carbon Monoxide 7U1EE4V452 Extracellular Signal-Regulated MAP Kinases EC 2.7.11.24 Mitogen-Activated Protein Kinases
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520 |a Carbon monoxide (CO), one of the haem oxygenase (HO) products, plays important roles in plant development and stress adaptation. However, the function of CO involved in wounding responses is seldom studied. A wound-inducible gene, ipomoelin (IPO), of sweet potato (Ipomoea batatas cv. Tainung 57) was used as a target to study the regulation of CO in wounding responses. After wounding for 1h, the endogenous CO content and IbHO expression level were significantly reduced in leaves. IPO expression upon wounding was prohibited by the HO activator hemin, whereas the HO inhibitor zinc protoporphyrin IX elevated IPO expression. The IPO expression induced by wounding, H2O2, or methyl jasmonate was inhibited by CO. CO also affected the activities of ascorbate peroxidase, catalase, and peroxidase, and largely decreased H2O2 content in leaves. CO inhibited the extracellular signal-regulated kinase (ERK) phosphorylation induced by wounding. IbMAPK, the ERK of sweet potato, was identified by immunoblotting, and the interaction with its upstream activator, IbMEK1, was further confirmed by bimolecular fluorescence complementation and co-immunoprecipitation. Conclusively, wounding in leaves repressed IbHO expression and CO production, induced H2O2 generation and ERK phosphorylation, and then stimulated IPO expression 
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650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a Carbon monoxide 
650 4 |a ERK phosphorylation 
650 4 |a H2O2 
650 4 |a ipomoelin 
650 4 |a sweet potato 
650 4 |a wounding. 
650 7 |a Plant Proteins  |2 NLM 
650 7 |a ipomoelin protein, Ipomoea batatas  |2 NLM 
650 7 |a Carbon Monoxide  |2 NLM 
650 7 |a 7U1EE4V452  |2 NLM 
650 7 |a Extracellular Signal-Regulated MAP Kinases  |2 NLM 
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650 7 |a Mitogen-Activated Protein Kinases  |2 NLM 
650 7 |a EC 2.7.11.24  |2 NLM 
700 1 |a Lin, Hsin-Hung  |e verfasserin  |4 aut 
700 1 |a Li, Yu-Chi  |e verfasserin  |4 aut 
700 1 |a King, Yu-Chi  |e verfasserin  |4 aut 
700 1 |a Sung, Ruei-Jin  |e verfasserin  |4 aut 
700 1 |a Kuo, Yun-Wei  |e verfasserin  |4 aut 
700 1 |a Lin, Chih-Ching  |e verfasserin  |4 aut 
700 1 |a Shen, Yu-Hsing  |e verfasserin  |4 aut 
700 1 |a Jeng, Shih-Tong  |e verfasserin  |4 aut 
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