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231224s2013 xx |||||o 00| ||eng c |
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|a 10.5713/ajas.2012.12406
|2 doi
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|a pubmed25n0801.xml
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|a DE-627
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|e rakwb
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|a eng
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| 100 |
1 |
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|a Kim, Eun Young
|e verfasserin
|4 aut
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| 245 |
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|a Effect of Glycosaminoglycans on In vitro Fertilizing Ability and In vitro Developmental Potential of Bovine Embryos
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|c 2013
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 22.07.2014
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|a Date Revised 21.10.2021
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|a published: Print
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|a Citation Status PubMed-not-MEDLINE
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|a The glycosaminoglycans (GAGs) present in the female reproductive tract promote sperm capacitation. When bovine sperm were exposed to 10 μg/ml of one of four GAGs (Chondroitin sulfate, CS; Dermatan sulfate, DS; Hyaluronic acid, HA; Heparin, HP) for 5 h, the total motility (TM), straight-line velocity (VSL), and curvilinear velocity (VCL) were higher in the HP- or HA-treated sperm, relative to control and CS- or DS-treated sperm. HP and HA treatments increased the levels of capacitated and acrosome-reacted sperm over time, compared to other treatment groups (p<0.05). In addition, sperm exposed to HP or HA for 1 h before IVF exhibited significantly improved fertilizing ability, as assessed by 2 pronucleus (PN) formation and cleavage rates at d 2. Exposure to these GAGs also enhanced in vitro embryo development rates and embryo quality, and increased the ICM and total blastocyst cell numbers at d 8 after IVF (p<0.05). A real-time PCR analysis showed that the expression levels of pluripotency (Oct 4), cell growth (Glut 5), and anti-apoptosis (Bax inhibitor) genes were significantly higher in embryos derived from HA- or HP-treated sperm than in control or other treatment groups, while pro-apoptotic gene expression (caspase-3) was significantly lower in all GAG treatment groups (p<0.05). These results demonstrated that exposure of bovine sperm to HP or HA positively correlates with in vitro fertilizing ability, in vitro embryo developmental potential, and embryonic gene expression
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|a Journal Article
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|a Capacitation
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|a Embryo Development
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|a Gene Expression
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|a Glycosaminoglycan
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| 650 |
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4 |
|a Heparin
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| 700 |
1 |
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|a Noh, Eun Hyung
|e verfasserin
|4 aut
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1 |
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|a Noh, Eun Ji
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Park, Min Jee
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Park, Hyo Young
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Lee, Dong Sun
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Riu, Key Zung
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Park, Se Pill
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Asian-Australasian journal of animal sciences
|d 1998
|g 26(2013), 2 vom: 24. Feb., Seite 178-88
|w (DE-627)NLM098195883
|x 1011-2367
|7 nnns
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|g volume:26
|g year:2013
|g number:2
|g day:24
|g month:02
|g pages:178-88
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|u http://dx.doi.org/10.5713/ajas.2012.12406
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