Identification of differentially expressed genes potentially involved in the tolerance of Lotus tenuis to long-term alkaline stress
Copyright © 2014 Elsevier Masson SAS. All rights reserved.
| Publié dans: | Plant physiology and biochemistry : PPB. - 1991. - 82(2014) vom: 15. Sept., Seite 279-88 |
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| Auteur principal: | |
| Autres auteurs: | , , , , , |
| Format: | Article en ligne |
| Langue: | English |
| Publié: |
2014
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| Accès à la collection: | Plant physiology and biochemistry : PPB |
| Sujets: | Journal Article Research Support, Non-U.S. Gov't Adaptive response Alkaline stress Long-term response Lotus tenuis Root and shoot tissues Stress induced transcripts Suppressive Subtractive Hybridization Antacids |
| Résumé: | Copyright © 2014 Elsevier Masson SAS. All rights reserved. Soil alkalinity is one of the most serious agricultural problems limiting crop yields. The legume Lotus tenuis is an important forage acknowledged by its ability to naturally grow in alkaline soils. To gain insight into the molecular responses that are activated by alkalinity in L. tenuis plants, subtractive cDNA libraries were generated from leaves and roots of these plants. Total RNAs of non-stressed plants (pH 5.8; E.C. 1.2), and plants stressed by the addition of 10 mM of NaHCO3 (pH 9.0; E.C. 1.9), were used as source of the driver and the tester samples, respectively. RNA samples were collected after 14 and 28 days of treatment. A total of 158 unigenes from leaves and 92 unigenes from roots were obtained and classified into 11 functional categories. Unigenes from these categories (4 for leaves and 8 for roots), that were related with nutrient metabolism and oxidative stress relief were selected, and their differential expression analyzed by qRT-PCR. These genes were found to be differentially expressed in a time dependent manner in L. tenuis during the alkaline stress application. Data generated from this study will contribute to the understanding of the general molecular mechanisms associated to plant tolerance under long-term alkaline stress in plants |
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| Description: | Date Completed 30.03.2015 Date Revised 30.09.2020 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1873-2690 |
| DOI: | 10.1016/j.plaphy.2014.06.009 |